SHUAI LIU1, JIANZHONG XU1,*, TINGSHAN LIU1, ZHIMING RAO2, WEIGUO ZHANG1,*
BIOCELL, Vol.46, No.10, pp. 2303-2313, 2022, DOI:10.32604/biocell.2022.020899
- 13 June 2022
Abstract In this study, phosphoenolpyruvate and erythrose-4-phosphate are efficiently supplied by collaborative design of Embden-Meyerhof-Parnas (EMP) pathway and pentose phosphate (PP) pathway in Escherichia coli, thus increasing the L-tryptophan production. Firstly, the effects of disrupting EMP pathway on L-tryptophan production were studied, and the results indicated that the strain with deletion of phosphofructokinase A (i.e., E. coli JW-5 ΔpfkA) produced 23.4 ± 2.1 g/L of L-tryptophan production. However, deletion of phosphofructokinase A and glucosephosphate isomerase is not conducive to glucose consumption and cell growth, especially deletion of glucosephosphate isomerase. Next, the carbon flux in PP pathway was enhanced by… More >
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