Gaoyin Wu^1,#, Yundou Pang^1,#, Ya Tan¹, Zhongcheng Peng¹, Shuanggui Geng¹, Guanglin Tao², Qiuying Li¹, Shuang Wang¹, Lihong Zhang¹, Quanyan Tian¹, Yingliang Liu^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.10, pp. 2597-2611, 2024, DOI:10.32604/phyton.2024.056875 - 30 October 2024

Abstract To establish an efficient regeneration method for the rare and endangered plant Mussaenda anomala to address problems regarding its reproductive obstacles and scarce populations. In this study, the terminal buds, axillary buds, stem segments with two axillary buds, stem segments with two axillary buds and one terminal bud, and leaves of M. anomala were used as explants. The effects of different explants and disinfection methods, plant growth regulators and substrates on plant regeneration were explored. The following results were obtained: (1) The terminal bud was a suitable explant for M. anomala tissue culture, and the disinfection method utilized… More >

Chaoying Wang¹, Lihui Ma², Yingzan Xie², Bo Ding³, Jingsi Huang⁴, Qian Wang^4,*

Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 1981-1993, 2024, DOI:10.32604/phyton.2024.050099 - 30 August 2024

Abstract Myricaria laxiflora, which grows along the Yangtze River in China, holds ornamental, ecological, and medicinal value. However, its wild population is threatened and currently designated protected as a national priority. The present research was the first to sequence and assemble M. laxiflora’s mitochondrial genome and examine its structural characteristics and phylogenetic relationships with other sequenced Caryophyllales species. The mitochondrial double-stranded closed-ring genome of M. laxiflora was found to be 389,949 bp in length, containing numerous repetitive sequences and RNA editing sites, with 34 protein encoding, 21 tRNA, and 3 rRNA genes. Although there are 22 fragments in the More >

XIAO-JUN ZHOU^1,*, YU-WEI TIAN¹, RUI-HAN LI²

BIOCELL, Vol.47, No.8, pp. 1907-1913, 2023, DOI:10.32604/biocell.2023.030191 - 28 August 2023

Abstract Background: Acer griseum Pax is an endangered species endemic to China with both ornamental and economic value. However, the lack of information on its genome size and characteristics hinders further work at the genome level. Methods: This paper applied bioinformatics methods to predict the characteristics and patterns of the A. griseum genome, providing an important basis for formulating its whole-genome sequencing scheme. This study also characterized the simple sequence repeats (SSRs) of A. griseum, laying the foundation for the development and application of genome-wide SSR markers. In this study, PE150 sequencing was performed by the BGI MGISEQ platform,… More >