XIAOXIA WANG^1,#, ZHONGYUAN YAO^1,2,#, DI LIU¹, CHUNHONG YU^3,*, HUI LI^1,3,*

BIOCELL, Vol.46, No.8, pp. 1803-1806, 2022, DOI:10.32604/biocell.2022.019731 - 22 April 2022

Abstract Each stage of embryonic development, including normal gamete maturation, fertilization, zygotic genome activation, and cleavage, is crucial for human reproduction. Early embryo arrest is a common phenomenon. It is estimated that about 40%–70% of human embryos are arrested at early developmental stages. However, the exact mechanism remains largely uncertain. Embryos can be investigated in vitro by way of the development of in vitro fertilization/intracytoplasmic sperm injection. In addition to iatrogenic factors related to abnormal oocyte/embryo development, multiple gene mutations have been found to be involved in such phenotypes. Based on the knowledge of known etiological factors, several More >

SITI NABILAH^1,2; TRI HANDOYO^2,3; KYUNG-MIN KIM⁴; MOHAMMAD UBAIDILLAH^2,*

BIOCELL, Vol.46, No.7, pp. 1633-1641, 2022, DOI:10.32604/biocell.2022.019111 - 17 March 2022

Abstract Somatic embryogenesis is an asexual reproduction process that occurs in many plant species, including rice. This process contains several totipotency markers such as Somatic Embryogenesis Receptor-like Kinase (SERK), Leafy Cotyledon1 (LEC1) and WUSCHEL-Related Homeobox4 (WOX4) and also a helpful model for embryo development and clones and transformations. Here, we report the gene expression during somatic embryo development correlates with regeneration frequency in 14 Javanica rice (pigmented and non-pigmented) using modifified N6 media supplemented with Kinetin (2.0 mg/L) and NAA (1.0 mg/L). Although there have been advances in understanding the genetic basis of somatic embryogenesis in… More >

BING QI¹, XINMING ZHENG², WENYONG LI³, QINGXIN WEI², QINGXUAN CHEN¹

BIOCELL, Vol.31, No.2, pp. 199-203, 2007, DOI:10.32604/biocell.2007.31.199

Abstract Although there is more evidence that shows that IFNs (interferons) plays a very important role in the early development of the embryo, the mechanism of IFNs is still unclear. Our study showed that IFRG is expressed from oocytes- through to the preimplantation embryo in rabbits. This finding provides some clues for better understanding the role of IFNs in the development of the embryo. The full length of rabbit IFRG cDNA (Accession No. AJ584672), with a 2794bp encoding 131 amino acid sequence, was cloned IFRG expression can be detected in 8 different tissues: ovary, heart, lung, More >

MARCOS S. DREON¹, HORACIO HERAS^1,2, RICARDO J. POLLERO¹

BIOCELL, Vol.30, Suppl.S, pp. 359-365, 2006

Abstract This article has no abstract. More >

M.T. TERUEL^1,2, R.C. CATALANO², J.A. CABODEVILA², S.S. CALLEJAS²

BIOCELL, Vol.29, No.2, pp. 183-186, 2005, DOI:10.32604/biocell.2005.29.183

Abstract Embryo development depends on maternal and embryonic factors. When occurs in vitro, embryos secrete factors that stimulate their development. The purpose of this study was to investigate the possible effects of embryos at morula stage on mouse embryo development in vitro. To obtain conditioned media (CM), morulas were cultured in groups of 5 (CM5) or 10 (CM10) in microdrops of Ham-F10 culture medium during 24 h and later they were removed. Subsequently, 365 morulas were cultured in CM5 and CM10 or in Ham-F10 media (as control group). No differences in blastocyst formation could be found between embryos… More >

MARÍA TERESA PONCE, MÓNICA ELIZABETH GUIÑAZÚ, RICARDO TIZIO

BIOCELL, Vol.26, No.2, pp. 263-266, 2002, DOI:10.32604/biocell.2002.26.263

Abstract The goal of this study was to determine the effect of putrescine, added to the culture medium, on the in vitro development of stenospermic grape (Vitis vinifera L) embryos. The cross breedings of Perlón x G.C88552 and Perlón x Argentina were used. 0 (control), 2 and 4 mM of putrescine were added to the immature seed’s culture medium. In Perlón x Argentina, 2mM of putrescine statistically increased the percentage of total embryos, direct germination, polyembryos and normal plants. In Perlón x G.C88552, only 2 mM of putrescine increased all the variables considered, eventually tripling the percentage of More >