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  • Open Access

    ARTICLE

    Physiological Response Mechanism and Drought Resistance Evaluation of Passiflora edulis Sims under Drought Stress

    Binyang Zhao1, Fengchan Wu2, Guojun Cai3, Peiyu Xi2, Yulin Guo2, Anding Li2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1345-1363, 2024, DOI:10.32604/phyton.2024.050950

    Abstract In order to explore the response mechanism of Passiflora edulis Sims to drought stress, the changes in morphological and physiological traits of Passiflora edulis Sims under different drought conditions were studied. A total of 7 germplasm resources of Passiflora edulis Sims were selected and tested under drought stress by the pot culture method under 4 treatment levels: 75%–80% (Control, CK) of maximum field water capacity, 55%–60% (Light Drought, LD) of maximum field water capacity, i.e., mild drought, 40%–45% (Moderate Drought, MD) of maximum field water capacity, i.e., moderate drought and 30%–35% (Severe Drought, SD) of maximum field water… More >

  • Open Access

    ARTICLE

    Oxysterol-Binding Protein-Related Protein 8 Inhibits Gastric Cancer Growth Through Induction of ER Stress, Inhibition of Wnt Signaling, and Activation of Apoptosis

    Xiaohe Guo*, Lanfang Zhang*, Yingying Fan*, Dezhong Zhang, Lei Qin*, Shuping Dong*, Guangyan Li*

    Oncology Research, Vol.25, No.5, pp. 799-808, 2017, DOI:10.3727/096504016X14783691306605

    Abstract Gastric cancer (GC) is the third leading cause of cancer-related mortality worldwide. Oxysterol-binding proteinrelated protein 8 (ORP8) functions as a sterol sensor that regulates a number of cellular functions. We showed that ORP8 expression was significantly lower in GC tissues and cells. Overexpression of ORP8 significantly inhibited GC cell proliferation in several GC cells. The formation of colonies in AGS cells was inhibited by the overexpression of ORP8. Moreover, overexpression of ORP8 significantly decreased implanted tumor growth in nude mice. Overexpression of ORP8 resulted in a significant increase in CHOP and GRP78 expression and the… More >

  • Open Access

    REVIEW

    Research Progress on Economic Forest Water Stress Based on Bibliometrics and Knowledge Graph

    Xin Yin1,#, Shuai Wang1,#, Chunguang Wang1, Haichao Wang2, Zheying Zong1,3,*, Zeyu Ban1

    Phyton-International Journal of Experimental Botany, Vol.93, No.5, pp. 843-858, 2024, DOI:10.32604/phyton.2024.049114

    Abstract This study employed the bibliometric software CiteSpace 6.1.R6 to analyze the correlation between thermal infrared, spectral remote sensing technology, and the estimation of economic forest water stress. It aimed to review the development and current status of this field, as well as to identify future research trends. A search was conducted on the China National Knowledge Infrastructure (CNKI) database using the keyword “water stress” for relevant studies from 2003 to 2023. The visual analysis function of CNKI was used to generate the distribution of annual publication volume, and CiteSpace 6.1.R6 was utilized to create network More >

  • Open Access

    ARTICLE

    Gp78 regulates PMP22 and causes ER stress and autophagy in EV71-VP1-overexpressing mouse Schwann cells

    DANPING ZHU1,#, GUANGMING LIU1,#, KUAN FENG1, SUYUN LI1, DANDAN HU2, SIDA YANG3,*, PEIQING LI1,*

    BIOCELL, Vol.48, No.4, pp. 653-664, 2024, DOI:10.32604/biocell.2024.044856

    Abstract Background: During Enterovirus type 71 (EV71) infection, the structural viral protein 1 (VP1) activates endoplasmic reticulum (ER) stress associated with peripheral myelin protein 22 (PMP22) accumulation and induces autophagy. However, the specific mechanism behind this process remains elusive. Methods: In this research, we used the VP1-overexpressing mouse Schwann cells (SCs) models co-transfected with a PMP22 silencing or Autocrine motility factor receptor (AMFR/gp78) overexpressing vector to explore the regulation of gp78 on PMP22 and its relationship with autophagy and apoptosis. Results: The activity of gp78 could be influenced by EV71-VP1, leading to a decrease in the ubiquitination… More >

  • Open Access

    ARTICLE

    Seed Priming and Foliar Supplementation with β-aminobutyric Acid Alleviates Drought Stress through Mitigation of Oxidative Stress and Enhancement of Antioxidant Defense in Linseed (Linum usitatissimum L.)

    Tauqeer Ahmad Yasir1,2, Muhammad Ateeq1,3, Allah Wasaya1,2,*, Mubshar Hussain2, Naeem Sarwar2, Khuram Mubeen4, Mudassir Aziz4, Muhammad Aamir Iqbal5, Chukwuma C. Ogbaga6, Ibrahim Al-Ashkar7, Md Atikur Rahman8, Ayman El Sabagh9,10,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3113-3131, 2023, DOI:10.32604/phyton.2023.029502

    Abstract Drought is one of the critical limitations to agricultural soils and crop plants. Scarcity of water is increasing due to climate change that lead to increasing threats to global food security. Therefore, ecofriendly and cost effective strategies are highly desirable for mitigating drought stress along with sustainable and smart agricultural production. The aim of the study was to mitigate DS using seed priming and exogenous supplementation of β-aminobutyric acid (BABA) in linseed (Linum usitatissimum L.). Different doses (0, 50, 100 and 150 µM) of BABA were used for seed priming agent and foliar spraying under three… More >

  • Open Access

    ARTICLE

    An Automatic Classification Grading of Spinach Seedlings Water Stress Based on N-MobileNetXt

    Yanlei Xu, Xue Cong, Yuting Zhai, Zhiyuan Gao, Helong Yu*

    Intelligent Automation & Soft Computing, Vol.37, No.3, pp. 3019-3037, 2023, DOI:10.32604/iasc.2023.040330

    Abstract To solve inefficient water stress classification of spinach seedlings under complex background, this study proposed an automatic classification method for the water stress level of spinach seedlings based on the N-MobileNetXt (NCAM+MobileNetXt) network. Firstly, this study reconstructed the Sandglass Block to effectively increase the model accuracy; secondly, this study introduced the group convolution module and a two-dimensional adaptive average pool, which can significantly compress the model parameters and enhance the model robustness separately; finally, this study innovatively proposed the Normalization-based Channel Attention Module (NCAM) to enhance the image features obviously. The experimental results showed that More >

  • Open Access

    ARTICLE

    Effect of Water Stress Induced by Polyethylene Glycol on Growth, Proline Accumulation in Agave americana L.

    Alfaro-Corres Arnoldo Enrique1,#, Lecona-Guzman Carlos Alberto1,#, Gutiérrez-Miceli Federico Antonio1, Martínez-Esteves Manuel2, Silverio-Gómez María del Carmen3, Medina-Lara María de Fátima2, Ruiz-Lau Nancy4,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 629-643, 2023, DOI:10.32604/phyton.2022.023970

    Abstract The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana L. In order to do the establishment of cells, the formation of callus was induced; for it two auxins were evaluated: 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-mino-3,5,6-trichloropicolinic acid (picloram) at three concentrations (0.25, 0.5 and 0.75 mg L−1) in three explants (leaf, root and meristems) cultured in MS semisolid medium. The callogenesis response was related to the type and section of the explant, as well as the regulator used, and a cell suspension was established… More >

  • Open Access

    ARTICLE

    Magnesium Demethylcantharidate induces apoptosis in hepatocellular carcinoma cells via ER stress

    XINTING ZHU1,2,#, MENG YE2,3,#, KELAN FANG1, FANG LIU1,2, JING HUI1,2, MEICHEN LIU2, XIAOFEI LI1,2, RONG YAN1,2,*, Yun Liu1,2,4,*

    BIOCELL, Vol.46, No.12, pp. 2595-2600, 2022, DOI:10.32604/biocell.2022.025468

    Abstract Cantharidin (CTD) is a bioactive ingredient isolated from Cantharis vesicatoria (blister beetles), which has potential therapeutic value as an anticancer agent. Magnesium Demethylcantharidate (MDC) is a recently developed derivative of Cantharidin (CTD), and previous studies have illustrated its excellent anticancer activity on HCC cells. However, the effect and mechanism of MDC remains unclear and need to be further studied. In particular, whether MDC can cause ER stress in HCC is still unknown. In this study, we demonstrated that endoplasmic reticulum stress (ERS)-related proteins were changed in SMMC-7721 and Bel-7402 cells after being exposed to MDC. Moreover, More >

  • Open Access

    ARTICLE

    Optimization of Callus Induction Conditions from Immature Embryos of Maize under Stress

    Xiaotong Wei1,#, Xuhong Fan2,#, Xuan Lu1, Liyuan Han1, Honglin Zhang3, Siyan Liu3, Jing Qu3, Shuyan Guan3,*, Yiyong Ma3,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1945-1957, 2022, DOI:10.32604/phyton.2022.020062

    Abstract

    The embryos of maize (Zea mays L.) inbred lines GS02, GS07, GS08, GS11 and GS15 were used as receptor materials to optimize the receptor system from the aspects of genotype, medium components and stress (PEG6000, mannitol, salt and low phosphorus). The results showed that GS07 had the highest induction rate (95.2%). Orthogonal test analysis showed that the best combination of medium components in induction was A2B3C1D3(2), namely, the concentration of 2, 4-dichlorophenoxy acetic acid (2,4-D) was 4 mg·mL-1, the concentration of L-Proline (L-Pro) was 0.8 mg·mL-1, and the concentration of silver nitrate (AgNO3) was 10 mg·mL-1 (or 5 mg·mL).

    More >

  • Open Access

    REVIEW

    ER exit pathways and the control of proteostasis: Crucial role of the UPR, COPII, and ER-phagy in the secretory pathway

    GIUSEPPINA AMODIO1, VALENTINA PAGLIARA1, PAOLO REMONDELLI1,*, ORNELLA MOLTEDO2

    BIOCELL, Vol.46, No.5, pp. 1131-1137, 2022, DOI:10.32604/biocell.2022.018638

    Abstract The endoplasmic reticulum (ER) is the site of entry of all proteins that function in the secretory pathway including the extracellular environment. Because it controls the folding of newly synthesized secretory proteins, the ER is indispensable for the maintenance of proteostasis in the secretory pathway. Within the ER and, in part, in post-ER compartments, the quality control of protein folding is under the regulation of the unfolded protein response (UPR) pathways. The UPR strategy is to enhance protein folding, increase the ER degradation pathway of misfolded proteins, and allow the exit from the ER of More >

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