QINGJIAN HE¹, HUIXIN ZHU^2,3, SHANHONG FANG^4,5,*

BIOCELL, Vol.48, No.2, pp. 205-216, 2024, DOI:10.32604/biocell.2023.045109 - 23 February 2024

Abstract Introduction: Dexamethasone (Dex) caused impaired osteoblast differentiation and oxidative stress (OS) in bone marrow mesenchymal stem cells (BMSCs). This work sought to elucidate the precise molecular pathway through which Dex influences osteogenic differentiation (OD) and OS in BMSCs. Methods: The expression of Runt-related transcription factor 1 (RUNX1) and alpha-2 macroglobulin (A2M) was assessed in Dex-treated BMSCs using qRT-PCR and Western Blot. Following the functional rescue experiments, cell proliferation was determined by MTT assay, reactive oxygen species (ROS) expression by DCFH-DA fluorescent probe, lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (Gpx) expression… More >

CHUNYANG XU^1,#, CAIYUN YANG^1,#, JINSONG ZHANG², XIAOHUA PAN³, JUN WANG⁴, LEI JIANG², HONGWEI YE^1,*, BO CHEN^1,*

BIOCELL, Vol.47, No.9, pp. 2027-2035, 2023, DOI:10.32604/biocell.2023.029277 - 28 September 2023

Abstract Background: The lung is one of the primary target organs of hydrogen sulfide (H₂S), as exposure to H₂S can cause acute lung injury (ALI) and pulmonary edema. Dexamethasone (Dex) exerts a protective effect on ALI caused by exposure to toxic gases and is commonly used in the clinic; however, the underlying mechanisms remain elusive, and the dose is unclear. Methods: In vivo experiments: divided C57BL6 mice into 6 groups at random, 12 in each group. The mice were exposed to H₂S for 3 h and 5 or 50 mg/kg Dex pretreated before exposure, sacrificed 12 h later. The… More >