Ya-Yi Chen^1,2, Tzu-Ting Chen³, Ya-Hsuan Chao⁴, Wen-Ho Chuo⁵, Chieh-Shan Wu^6,7, Ruo-Han Tseng^8,*, Chieh-Chen Huang^9,*

BIOCELL, Vol.49, No.9, pp. 1733-1748, 2025, DOI:10.32604/biocell.2025.067011 - 25 September 2025

Abstract Objectives: Professional antigen-presenting cells known as dendritic cells (DCs) assist as a connection between the innate and adaptive components of the immune response. DCs are attractive targets for immunomodulatory drugs because of their crucial function in triggering immunity. This study set out to examine, for the first time, how hibifolin affected mouse bone-marrow derived (BMDCs) dendritic cells, triggered by lipopolysaccharide (LPS) in vitro. Additionally, a mouse model of contact hypersensitivity (CHS) was used to assess its possible therapeutic effects in vivo. Methods: LPS was administered to BMDCs with or without hibifolin. Major Histocompatibility Complex (MHC) class II,… More >

JIAJIA LV, XIAOYOU ZHONG, LIN WANG, WEIFEI FAN^*

Oncology Research, Vol.33, No.7, pp. 1581-1592, 2025, DOI:10.32604/or.2025.060063 - 26 June 2025

Abstract The tumor microenvironment (TME) is a complex and dynamic network comprised of tumor cells, surrounding cellular components, various signaling molecules, and the stroma. Myeloid-derived suppressor cells (MDSCs) are pivotal players in the immunosuppressive landscape of the TME, effectively hindering antitumor immune responses and facilitating tumor progression. Originating from pathologically activated myeloid precursors and relatively immature myeloid cells, MDSCs retain plasticity to further differentiate into other myeloid cells, such as macrophages or dendritic cells, which underpins their heterogeneity and adaptability in response to the TME. In this review, we delve into the plasticity of MDSCs in More >

Yahui Wu^1,2, Tiansheng Dai¹, Jingwen Qin^1,2, Jian Guo^1,2, Jitao Fan^1,2, Jun Mei^1,2, Xiaoli Li^1,2, Fang Liu^1,2,*

BIOCELL, Vol.49, No.4, pp. 701-720, 2025, DOI:10.32604/biocell.2025.061289 - 30 April 2025

Abstract Background: Dendritic cells (DCs) play a pivotal role in antigen presentation and regulating adaptive immune responses in asthma pathophysiology. However, the underlying molecular mechanisms remain incompletely understood. Methods: Bioinformatics analysis of the GSE27011 dataset identified differentially expressed genes associated with pediatric asthma. An ovalbumin (OVA)-induced asthma mouse model and an Rfx5 knockdown model were established. RFX5 expression was assessed in DCs from patients with asthma and asthmatic mouse lung tissues using qRT-PCR, Western blotting, and immunohistochemistry. The regulatory effects of regulatory factor X5 (RFX5) on histone deacetylase 2 (HDAC2), class II major histocompatibility complex transactivator… More >

JYOTHI B. NAIR^1,2, ANU MARY JOSEPH³, SANOOP P.⁴, MANU M. JOSEPH^5,*

BIOCELL, Vol.48, No.11, pp. 1579-1602, 2024, DOI:10.32604/biocell.2024.054879 - 07 November 2024

Abstract Nanoparticles represent a heterogeneous collection of materials, whether natural or synthetic, with dimensions aligning in the nanoscale. Because of their intense manifestation with the immune system, they can be harvested for numerous bio-medical and biotechnological advancements mainly in cancer treatment. This review article aims to scrutinize various types of nanoparticles that interact differently with immune cells like macrophages, dendritic cells, T lymphocytes, and natural killer (NK) cells. It also underscores the importance of knowing how nanoparticles influence immune cell functions, such as the production of cytokines and the presentation of antigens which are crucial for… More >

LEI ZHONG¹, LEI GUO¹, ZHISHUAI YE², SHANFENG ZHANG³, RONGCHONG HUANG^2,*

BIOCELL, Vol.44, No.2, pp. 225-236, 2020, DOI:10.32604/biocell.2020.08714 - 27 May 2020

Abstract Studies have found that a large number of inflammatory cells, P-selectin, and mature dendritic cells (DCs) are expressed in the damaged and shoulder parts of atherosclerotic plaque, which demonstrates that P-selectin and mature DCs participate in the immune inflammatory response leading to the development of atherosclerosis. However, it is unclear how the above factors interact in this setting. In this study, we investigated the role of P-selectin and its receptor, P-selectin glycoprotein ligand (PSGL)-1 in atherosclerosis, with the finding that DC surface marker expression was consistently high in the P-selectin group while consistently low in More >

Anastasia S. Proskurina¹, Alisa V. Spaselnikova^1,2, Genrikh S. Ritter^1,2, Evgenia V. Dolgova¹, Ekaterina A. Potter¹, Margarita V. Romanenko², Sergey V. Netesov², Yaroslav R. Efremov^1,2, Oleg S. Taranov³, Nikolay A. Varaksin⁴, Tatiana G. Ryabicheva⁴, Aleksandr A. Ostanin⁵, Elena R. Chernykh⁵, Sergey S. Bogachev¹

European Cytokine Network, Vol.30, No.2, pp. 43-58, 2019, DOI:10.1684/ecn.2019.0427

Abstract The present study demonstrates that monocyte-derived dendritic cells (moDCs) produced in vitro using a GM-CSF and IFN-α differentiation protocol encompass a rare (~5%) subpopulation of cells showing classical dendritic cell morphology and capable of natural internalization of extracellular self-DNA. We established that DEFB, HMGB1, LL-37 and RAGE antigens, which mediate the process of DNA internalization, are expressed on the surface of moDCs similar to plasmacytoid dendritic cells. However, in constrast to the latter subpopulation, these cells do not produce interleukin (IL)-37. Nonetheless, the process of DNA internalization was not in direct relation to the presence… More >

Zhu Zeng^1,*, Zuquan Hu¹, Qinni Zheng¹, Xiaoli Xu¹, Rong Dong¹, Hui Xue¹, Hui Yang¹

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 68-69, 2019, DOI:10.32604/mcb.2019.07085

Abstract Dendritic cells (DCs) play a crucial role in initiating and amplifying both the innate and adaptive immune responses [1]. Clinically, the DCs-based immunotherapy against cancer is considered one of the most promising therapies to overcome cancers, but there are still many challenges need to be overcome [2]. The motility of DCs is especially crucial for migration of immature DCs into peripheral tissue and dynamic physical interaction between mature DCs and naive T cells in the secondary lymph node. This study focuses on the investigations of DCs at different differentiation stages and under various suppressive cytokines… More >

Wenhui Hu¹, Yun Wang¹, Jin Chen¹, Yonggang Song¹, Jinhua Long¹, Zhu Zeng^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 67-67, 2019, DOI:10.32604/mcb.2019.07083

Abstract The responses of dendritic cells (DCs) to the mechanical microenvironment caused by implanted materials are highly correlated to the host immune responses and largely determines the outcome of tissue regeneration [1,2]. In the early stage of the inflammations following injury or implantation, a large amount of fibrin would deposit around the implanted materials and form a microporous fibrous-liked network structure, which can provide mechanical microenvironment with different spatial confinement in dimensions for following recruited DCs. Herein, we have established a useful model by salmon fibrin to mimic the deposited fibrin matrix and found that DCs More >

Fuzhou Tang¹, Jin Chen¹, Shichao Zhang¹, Zuquan Hu¹, Lina Liu¹, Long Li¹, Yan Ouyang¹, Zhu Zeng^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 40-41, 2019, DOI:10.32604/mcb.2019.07082

Abstract As potent antigen presenting cells, dendritic cells (DCs) are utilized to deliver the signals essential for the initiation of immune responses. The motility of DCs is crucial for migration of immature DCs (imDCs) in peripheral tissue and the interaction between mature DCs (mDCs) and naïve T cells in the secondary lymph node. From biomechanical viewpoint, the deformability of cells is necessary for their motility. Deformation of cells can be divided into active deformation (e.g. chemotaxis) and passive deformation (e.g. migration under shear stress of blood flow). However, there is no detailed study on the deformability More >

Molecular & Cellular Biomechanics, Vol.15, No.1, pp. 21-36, 2018, DOI:10.3970/mcb.2018.015.021

Abstract Dendritic cells (DCs) are the most potent professional antigen presenting cells as now known, which play critical roles in the initiation, programming and regulation of the immune response. Transforming growth factor-β₁ (TGF-β₁), one of the major suppressive cytokines in tumor microenvironment, can deteriorate the biomechanical characteristics and motility of mature dendritic cells (mDCs)，but the underlying molecular mechanisms are not well defined. In this study, the effects of TGF-β₁ on the motilities and T cell priming capabilities of mDCs as well as the molecular regulatory mechanisms were investigated. The results showed that the cytoskeleton (F-actin) organizations of mDCs More >