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    ARTICLE

    Evaluation of Mechanical and Chemical Stimulations on Osteocalcin and Runx2 Expression in Mesenchymal Stem Cells

    Maryam Jazayeri1, Mohammad Ali Shokrgozar1, Nooshin Haghighipour1,2, Reza Mahdian3, Mehdi Farrokhi1, Shahin Bonakdar1, FereshtehMirahmadi1, Tannaz Nourizadeh Abbariki

    Molecular & Cellular Biomechanics, Vol.12, No.3, pp. 197-213, 2015, DOI:10.3970/mcb.2015.012.197

    Abstract The osseous tissue repair and regeneration have great importance in orthopedic and maxillofacial surgery. Tissue engineering makes it possible to cure different tissue abnormalities using autologous grafts. It is now obvious that mechanical loading has essential role in directing cells to differentiation. In this study, the influence of cyclic uniaxial loading and its combination with chemical factors on expression of osteogenic markers was investigated. Rat bone marrow-derived stem cells were isolated and cultured. In one group cells were maintained in chemical induction medium. In another group cells were subjected to cyclic uniaxial strain with 3%… More >

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