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    ARTICLE

    Study of phylogenic relationships of some wild and crop species of Iranian Crocus by ITS nuclear loci

    Farshid GOLSHANI1, Barat Ali FAKHERI1, Mahmood SOLOUKI1, Nafiseh MAHDINEZHAD1, Majid Reza KIANI FERIZ2

    BIOCELL, Vol.43, No.3, pp. 225-232, 2019, DOI:10.32604/biocell.2019.07547

    Abstract ITS (Internal Transcription Spacer of nuclear ribosomal DNA) regions method was applied to identify and study the phylogenetic relationships existing between 41 individuals of Crocus, including 8 species collected in Iran (4 crop and 8 wild individuals) and 16 species (2 crop and 27 wild individuals) already registered in GenBank (NCBI). PCR amplifications were performed using the primer designed on the nucleotide sequence of nuclear region (ITS). A total of 12 sequences were obtained and registered in NCBI database. In particular, 4 of these sequences were not already present in the scientific library. Nucleotide… More >

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