ARMAND N. YAZDANI¹, MICHAELA PLETSCH¹, ABRAHAM CHORBAJIAN¹, DAVID ZITSER¹, VIKRANT RAI^1,2,*

BIOCELL, Vol.48, No.12, pp. 1683-1702, 2024, DOI:10.32604/biocell.2024.056252 - 30 December 2024

Abstract Mixed cholangiocarcinoma is a rare and aggressive neoplastic proliferation of biliary tract epithelial cells, accounting for up to 20% of primary liver cancers. It is the second most common primary liver malignancy with a 5-year survivability of less than 10% at diagnosis and is associated with various inflammatory diseases. Current management involves systemic chemotherapy, targeted radiation, and surgical resection, but long-term survival remains low, especially for surgically unresectable cases. Novel discoveries and understandings of the tumor microenvironment reveal new opportunities for targeted therapies for cholangiocarcinoma. Specifically, new pharmaceuticals including cell-based vaccines, tumor-associated neutrophils, and hepatic… More >

YUSEN ZHANG^1,2,3, LIPING LIU^1,2,3, BIWEI LUO^1,2,3, HONGGUI TANG^1,2,3, XIAOFANG YU^1,2,3, SHIYUN BAO^1,2,3,*

Oncology Research, Vol.31, No.3, pp. 317-331, 2023, DOI:10.32604/or.2023.028418 - 22 May 2023

Abstract Background: Cholangiocarcinoma (CCA) represents the epithelial cell cancer with high aggressiveness whose five-year survival rate is poor with standard treatment. Calcyclin-binding protein (CACYBP) shows aberrant expression within several malignant tumors, but the role of CACYBP in CCA remains unknown. Methods: Immunohistochemical (IHC) analysis was used to identify CACYBP overexpression in clinical samples of CCA patients. Moreover, its correlation with clinical outcome was revealed. Furthermore, CACYBP’s effect on CCA cell growth and invasion was investigated in vitro and in vivo using loss-of-function experiments. Results: CACYBP showed up-regulation in CCA, which predicts the dismal prognostic outcome. CACYBP had an important effect More >

Yi Chen^1,#, Liyun Huang^1,#, Zuwu Wei¹, Xiaoling Liu¹, Lihong Chen^1,2,*, Bin Wang^1,2,*

Oncologie, Vol.24, No.2, pp. 329-340, 2022, DOI:10.32604/oncologie.2022.022521 - 29 June 2022

Abstract Background: The effective method for predicting prognosis of ICC is still lack. This study aims to establish and verify an effective prognostic nomogram model for intrahepatic cholangiocarcinoma (ICC) after partial hepatectomy. Materials and Methods: A nomogram model was developed in a cohort of 127 patients from January 2015 to December 2019. General clinical characteristics including preoperative physical examination data and postoperative pathological features were obtained. The independent risk factors identified by univariate and multivariate COX proportional hazards regression models were used to construct nomogram model. Predictive accuracy and discriminative ability were determined using a concordance index and… More >

Ming Zhang, Baochang Shi, Kai Zhang

Oncology Research, Vol.27, No.9, pp. 1061-1068, 2019, DOI:10.3727/096504019X15565325878380

Abstract Deregulation of miR-186 and Twist1 has been identified to be involved in the progression of multiple cancers. However, the detailed molecular mechanisms underlying miR-186-involved cholangiocarcinoma (CCA) are still unknown. In this study, we found that miR-186 was downregulated in CCA tissues and cell lines, and negatively correlated with the expression of Twist1 protein. In vitro assays demonstrated that miR-186 mimics repressed cell proliferation, in vivo tumor formation, and caused cell cycle arrest. miR-186 mimics also inhibited the migration and invasion of CCLP1 and SG-231 cells. Mechanistically, the 3′-untranslated region (3′-UTR) of Twist1 mRNA is a More >

Zhen Li^*1, Xin Li^*1, Xiao Du^†, Henghui Zhang^†, Zhengyang Wu^*, Kewei Ren^*, Xinwei Han^*

Oncology Research, Vol.27, No.6, pp. 663-672, 2019, DOI:10.3727/096504018X15420741307616

Abstract Cholangiocarcinoma (CCA) is the second most common primary hepatobiliary carcinoma. The long noncoding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) has been reported to contribute to the progression of multiple cancers. Nonetheless, the functions and hidden mechanism of SNHG1 remain unclear in CCA. In this study, the SNHG1 levels were boosted in CCA cell lines, and knockdown of SNHG1 repressed CCA cell proliferation and invasion in vitro. The data also demonstrated that miR-140 could act as a target of SNHG1 in CCA and inhibited CCA cell proliferation and invasion, whereas the inhibition effects More >

Lunjian Chen^*, Xiaorong Huang^†, Xinxin Chen^‡

Oncology Research, Vol.26, No.9, pp. 1375-1382, 2018, DOI:10.3727/096504018X15188352857437

Abstract Cholangiocarcinoma (CCA) is one of the most malignant adenocarcinomas arising from bile duct epithelial cells. However, the molecular mechanism regulating CCA development and progression still needs to be investigated. Here we found that miR-365 was downregulated in CCA tissues compared with adjacent normal tissues. By functional experiments, we found that overexpression of miR-365 significantly inhibited CCA cell proliferation and promoted cellular apoptosis in vitro. Furthermore, administration with miR-365 markedly suppressed the growth of tumor tissues in vivo. Mechanistically, we identified E2F2 as the target gene of miR- 365 in CCA cells. We found that overexpression More >

Cheng Zhang^*, Jing-Yi Li^*, Fu-Zhou Tian^†, Gang Zhao^‡, Hai Hu^‡, Yue-Feng Ma^*, Yu-Long Yang^*

Oncology Research, Vol.26, No.6, pp. 879-888, 2018, DOI:10.3727/096504017X15024935181289

Abstract Long noncoding RNAs (lncRNAs) are known to play important roles in cancers. However, little is known about lncRNAs in cholangiocarcinoma (CCA), a cholangiocyte malignancy with poor prognosis. We investigated the role of nuclear paraspeckle assembly transcript 1 (NEAT1) lncRNA in promoting CCA. qRT-PCR analysis of patient samples showed that NEAT1 expression was higher in CCA tumors than in matched adjacent nontumor tissue. NEAT1 levels were also higher in CCA cell lines than in a normal biliary epithelium cell line (HIBEpic). NEAT1 knockdown in CCA cell lines using shNEAT1 reduced cell proliferation and colony formation in… More >

Waraporn Saentaweesuk^*†‡, Norie Araki^‡, Kulthida Vaeteewoottacharn^*†, Atit Silsirivanit^*†‡, Wunchana Seubwai^†§, Chutima Talabnin^¶, Kanha Muisuk^§, Banchob Sripa^†#, Sopit Wongkham^*†, Seiji Okada^**, Chaisiri Wongkham^*†

Oncology Research, Vol.26, No.4, pp. 605-616, 2018, DOI:10.3727/096504017X15009778205068

Abstract Cholangiocarcinoma (CCA) is a highly metastatic tumor, and the majority of patients with CCA have a short survival time because there are no available effective treatments. Hence, a better understanding regarding CCA metastasis may provide an opportunity to improve the strategies for treatment. A comparison study between the highly metastatic cells and their parental cells is an approach to uncover the molecular mechanisms underlying the metastatic process. In the present study, a lung metastatic CCA cell line, KKU-214L5, was established by the in vivo selection of the tail vein-injected mouse model. KKU-214L5 cells possessed mesenchymal… More >

Paweena Dana^*†‡, Ryusho Kariya^‡, KulthidaVaeteewoottacharn^*†, Kanlayanee Sawanyawisuth^*†, Wunchana Seubwai^†§, Kouki Matsuda^‡, Seiji Okada^‡, Sopit Wongkham^*†

Oncology Research, Vol.25, No.7, pp. 1047-1059, 2017, DOI:10.3727/096504016X14813899000565

Abstract CD147 is a transmembrane protein that can induce the expression and activity of matrix metalloproteinases (MMPs). Expression of CD147 has been shown to potentiate cell migration, invasion, and metastasis of cancer. In this study, the critical role of CD147 in metastasis was elucidated using CD147-overexpressing cholangiocarcinoma (CCA) cells in vitro and in vivo. The molecular mechanism, demonstrated herein, supported the hypothesis that metastasis increased in CD147-overexpressing cells. Five CD147-overexpressing clones (Ex-CD147) were established from a low CD147-expressing CCA cell line, KKU-055, using lentivirus containing pReceiver-Lenti-CD147. The metastatic capability was determined using the tail vein injection… More >

Wunchana Seubwai^*†‡, Kulthida Vaeteewoottacharn^‡§, Ratthaphol Kraiklang^¶, Kazuo Umezawa^#, Seiji Okada^**, Sopit Wongkham^‡§

Oncology Research, Vol.23, No.1-2, pp. 21-28, 2015, DOI:10.3727/096504015X14424348426071

Abstract Cholangiocarcinoma (CCA) is a dismal cancer. At present, there is no effective chemotherapeutic regimen for CCA. This may be due to the marked resistance of CCA to chemotherapy drugs, for which a mechanism remains unknown. Nuclear factor-kB (NF-kB) is constitutively activated in a variety of cancer cells, including CCA. It has been shown to play roles in growth, metastasis, and chemoresistance of cancer. In the present study, we examined whether NF-kB is involved in the chemoresistance of CCA and whether dehydroxymethylepoxyquinomicin (DHMEQ), an effective NF-kB inhibitor, can overcome the drug resistance of CCA. Two CCA… More >