Anastasia S. Proskurina¹, Alisa V. Spaselnikova^1,2, Genrikh S. Ritter^1,2, Evgenia V. Dolgova¹, Ekaterina A. Potter¹, Margarita V. Romanenko², Sergey V. Netesov², Yaroslav R. Efremov^1,2, Oleg S. Taranov³, Nikolay A. Varaksin⁴, Tatiana G. Ryabicheva⁴, Aleksandr A. Ostanin⁵, Elena R. Chernykh⁵, Sergey S. Bogachev¹

European Cytokine Network, Vol.30, No.2, pp. 43-58, 2019, DOI:10.1684/ecn.2019.0427

Abstract The present study demonstrates that monocyte-derived dendritic cells (moDCs) produced in vitro using a GM-CSF and IFN-α differentiation protocol encompass a rare (~5%) subpopulation of cells showing classical dendritic cell morphology and capable of natural internalization of extracellular self-DNA. We established that DEFB, HMGB1, LL-37 and RAGE antigens, which mediate the process of DNA internalization, are expressed on the surface of moDCs similar to plasmacytoid dendritic cells. However, in constrast to the latter subpopulation, these cells do not produce interleukin (IL)-37. Nonetheless, the process of DNA internalization was not in direct relation to the presence… More >

Pei Yin^*1, Jinpeng Jia^†1, Jijun Li^*, Yan Song^*, Yiyan Zhang^*, Fengkun Chen^*

Oncology Research, Vol.24, No.1, pp. 65-72, 2016, DOI:10.3727/096504016X14587366983838

Abstract Renal cell carcinoma (RCC) is the most common malignancy in the kidney in the world, and the 5-year overall survival for patients remains poor due to the lack of effective treatment strategies. Although ABT-737, as a Bcl-2/Bcl-xL inhibitor, has recently emerged as a novel cancer therapeutic reagent, apoptosis induced by ABT-737 is often blocked in several types of cancer cells. This study investigated whether the combination of the small-molecule BH3 mimetic ABT-737 and the lysosome inhibitor chloroquine was an effective strategy for treating renal cancer cells. We found that the combination of ABT-737 and chloroquine… More >

IVÓN TERESA NOVAK, HUMBERTO RAMÓN CABRAL

BIOCELL, Vol.32, No.2, pp. 169-174, 2008, DOI:10.32604/biocell.2008.32.169

Abstract We had previously found in autologous human leukocyte cultures, in which dead neutrophils phagocytosis by macrophages occur, macrophages and T CD4 lymphocytes perform a selective cell-cell interaction showing many figures of either one, two or several T- lymphocytes adhering to a central macrophage were seen. Considering that antigen presentation would be necessary for the formation of these immune synapses, we attempted to block rosette formation (i.e., the formation of macrophage associations with at least three lymphocytes) by interfering with both antigen processing and presentation. Culture samples of autologous leukocytes from 7 healthy donors were subjected More >