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  • Open Access

    REVIEW

    Microfluidic platform for circulating tumor cells isolation and detection

    JIAHAO ZHANG1,2,3, JIE REN4, ZIRUI LI1,2,3,*, YIXING GOU1,2,3,*

    BIOCELL, Vol.47, No.7, pp. 1439-1447, 2023, DOI:10.32604/biocell.2023.028628 - 21 June 2023

    Abstract Circulating tumor cells (CTCs) are essential biomarkers for liquid biopsies, which are important in the early screening, prognosis, and real-time monitoring of cancer. However, CTCs are less abundant in the peripheral blood of patients, therefore, their isolation is necessary. Recently, the use of microfluidics for CTC sorting has become a research hotspot owing to its low cost, ease of integration, low sample consumption, and unique advantages in the manipulation of micron-sized particles. Herein, we review the latest research on microfluidics-based CTC sorting. Specifically, we consider active sorting using external fields (electric, magnetic, acoustic, and optical More >

  • Open Access

    ARTICLE

    Development of a cytokine-secreting-based assay for the identification, sorting and transcriptomic analysis of polyfunctional human T cells

    Julie G. Burel1,2, Simon H. Apte1, Denise L. Doolan1,2

    European Cytokine Network, Vol.26, No.4, pp. 67-72, 2015, DOI:10.1684/ecn.2015.0369

    Abstract Polyfunctional T cells that simultaneously produce the cytokines IFN-γ, IL-2 and TNF have been correlated with better clinical outcomes in various diseases. To date, cytokine polyfunctionality within T cells has been exclusively studied by intracellular cytokine staining coupled with flow cytometric analysis. Thus, further downstream interrogation of polyfunctional T cell characteristics such as transcriptomic analysis has not been possible. Here, we report the use of a flow cytometric method based on cytokine secretion assay technology to detect and isolate, for the first time, viable human polyfunctional T cells directly from in vitro stimulated whole blood More >

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