Ye Lou^*1, Lei Liu^†1, Lihui Zhan^‡, Xuewei Wang^§, Hua Fan^¶

Oncology Research, Vol.24, No.2, pp. 89-97, 2016, DOI:10.3727/096504016X14597766487753

Abstract Acute lymphoblastic leukemia (ALL) is the most common childhood malignancy and causes a high rate of mortality in affected adults. Many subtypes of ALL exist with disruptions in distinct genetic pathways, including those regulated by miRNAs. Here we identify miR-187-5p as being highly upregulated in B-cell ALL and a driver of cellular proliferation and suppressor of apoptosis. We show that miR-187-5p directly targets the 3'-UTR of DKK2 to mediate these effects. We further determine that inhibition of DKK2 by miR-187-5p in Nalm-6 B cells leads to inappropriate activation of Wnt/β-catenin signaling. Together, these findings reveal More >

Yangjing Chen, Shaoqiang Zhang, Ruimin Zhao, Qian Zhao, Ting Zhang

Oncology Research, Vol.25, No.8, pp. 1215-1222, 2017, DOI:10.3727/096504016X14791715355957

Abstract Medullary thyroid carcinoma (MTC) is a neuroendocrine cancer derived from parafollicular C cells in the thyroid gland. It has great interest as a research focus because of its unusual genetic, clinical, and prognostic characteristics. However, the pathogenesis in MTC is not completely clear. We investigated the role of miR- 9-3p and bladder cancer-associated protein (BLCAP) in MTC TT cells. First, miR-9-3p expression was upregulated in human MTC tissues and TT cells and compared to the control by RT-PCR. Flow cytometric analysis indicated that the cell cycle progression in TT cells was significantly inhibited by the… More >

Yi-Bin Meng, Xin He, Yun-Fei Huang, Qi-Ning Wu, Yong-Cun Zhou, Ding-Jun Hao

Oncology Research, Vol.25, No.7, pp. 1207-1214, 2017, DOI:10.3727/096504017X14886679715637

Abstract It has been determined that long noncoding RNAs (lncRNAs) are identified as a potential regulatory factor in multiple tumors as well as multiple myeloma (MM). However, the role of colorectal neoplasia differentially expressed (CRNDE) in the pathogenesis of MM remains unclear. In this study, we found that the CRNDE expression level, in MM samples and cell lines, is higher than that in the control detected by real-time qPCR, which is also closely related to tumor progression and poor survival in MM patients. Knockdown of CRNDE significantly inhibits the proliferative vitality of MM cells (U266 and… More >

Yan Zhen^*1, Jia Liu^*†1, Yujie Huang^*†1, Yajun Wang^*, Wen Li^*†, Jun Wu^*†

Oncology Research, Vol.25, No.7, pp. 1109-1116, 2017, DOI:10.3727/096504016X14800889609439

Abstract Although increasing evidence indicates that the deregulation of microRNAs (miRNAs) contributes to tumorigenesis and invasion, little is known about the role of miR-133b in human non-small cell lung cancer (NSCLC). In the present study, we revealed that the introduction of miR-133b dramatically suppressed NSCLC cell growth, migration, and invasion in vitro. On the contrary, miR-133b inhibitors promoted cell growth, migration, and invasion in vitro. Further studies revealed that matrix metallopeptidase 9 (MMP9) is a direct target gene of miR-133b. Silencing MMP9 inhibited cell growth, migration, and invasion of NSCLC cells, which was consistent with the More >

Sheqing Ji, Bin Zhang, Ye Kong, Fei Ma, Yawei Hua

Oncology Research, Vol.25, No.6, pp. 853-861, 2017, DOI:10.3727/096504016X14759582767486

Abstract MicroRNAs (miRNAs) play a crucial role in the development and progression of human cancers, including gastric cancer (GC). The discovery of miRNAs may provide a new and powerful tool for studying the mechanism, diagnosis, and treatment of GC. In this study, we aimed to investigate the role of miR-326 in the development and progression of GC. Quantitative PCR (qPCR) was used to measure the expression level of miR-326 in GC tissues and cell lines. We found that miR-326 was significantly downregulated during GC. In addition, overexpression of miR-326 inhibited GC cell proliferation. Fluorescence-activated cell sorting More >

Kexin Lou^*1, Ning Chen^†1, Zhihong Li^*, Bei Zhang^†, Xiuli Wang^‡, Ye Chen^*, Haining Xu^*, Dongwei Wang^*, Hao Wang^*

Oncology Research, Vol.25, No.1, pp. 65-73, 2017, DOI:10.3727/096504016X14719078133366

Abstract Abnormal expression of microRNA (miR)-142-5p has been reported in hepatocellular carcinoma (HCC). However, little information is available regarding the functional role of miR-142-5p in HCC. We aimed to explore the effects of miR-142-5p aberrant expression on HCC cell growth and cell apoptosis, as well as the underlying mechanism. Human HCC cell lines HepG2 and SMMC-7721 cells were transfected with miR- 142-5p mimic, inhibitor, or a corresponding negative control. Cell viability, cell cycle distribution, and cell apoptosis were then analyzed. In addition, protein expression of Forkhead box, class O (FOXO) 1 and 3, a Bcl-2-interacting mediator… More >

Jie Shen^*, Jianhua Zhang^†, Minhui Xiao^*, Junfeng Yang^*, Ningnan Zhang^*

Oncology Research, Vol.26, No.8, pp. 1155-1165, 2018, DOI:10.3727/096504017X15041934685237

Abstract miR-203 is an epigenetically silenced tumor-suppressive microRNA in tumors. This study was designed to investigate the effects of miR-203 on the proliferation, migration, invasion, and apoptosis of bladder cancer (BCa) cells. The expression levels of miR-203 in BCa tissues, normal adjacent tissues, and BCa cell lines were detected. BCa cells were transfected with miR-203 mimic and inhibitor to investigate the effect of miR-203 on cell functions and the epithelial–mesenchymal transition (EMT). Cotransfection with miR-203 inhibitor and shRNA of the predicted target gene Twist1 (si-Twist1) was performed to investigate the target relationship of miR-203 and Twist1.… More >

Yifan Lian^*1, Weiming Fan^†1, Yanlin Huang^‡, Hongbo Wang^*, Jialiang Wang^*, Liang Zhou^‡, Xiaojuan Wu^‡, Meihai Deng^†, Yuehua Huang^*‡

Oncology Research, Vol.26, No.5, pp. 691-701, 2018, DOI:10.3727/096504017X15101398724809

Abstract Trophinin-associated protein (TROAP) was a protein first identified to mediate the process of embryo transplantation and later found to be involved in microtubule regulation. However, little is known about the role of TROAP in hepatocellular carcinoma (HCC). In the present study, we reported that both TROAP mRNA and protein expressions were downregulated in human HCC samples as well as cell lines. A high level of TROAP was associated with small tumor size (p<0.05), minor tumor nodules (p<0.01), and mild vein invasion (p<0.05). We further constructed in vitro TROAP depletion and overexpression HCC cell models. TROAP depletion significantly More >

Dandan Li^*, Changjun He^†, Junfeng Wang^†, Yanbo Wang^†, Jianlong Bu^†, Xianglong Kong^†, Dawei Sun^†

Oncology Research, Vol.26, No.3, pp. 385-400, 2018, DOI:10.3727/096504017X14973124850905

Abstract Many studies have shown that downregulation of miR-138 occurs in a variety of cancers including non-small cell lung cancer (NSCLC). However, the precise mechanisms of miR-138 in NSCLC have not been well clarified. In this study, we investigated the biological functions and molecular mechanisms of miR-138 in NSCLC cell lines, discussing whether it could turn out to be a therapeutic biomarker of NSCLC in the future. In our study, we found that miR-138 is downregulated in NSCLC tissues and cell lines. Moreover, the low level of miR-138 was associated with increased expression of SOX4 in… More >

Anne Riemann^*, Antje Güttler^†, Verena Haupt^*, Henri Wichmann^†, Sarah Reime^*, Matthias Bache^†, Dirk Vordermark^†, Oliver Thews^*

Oncology Research, Vol.26, No.2, pp. 191-200, 2018, DOI:10.3727/096504017X14965111926391

Abstract Carbonic anhydrase (CA) IX has emerged as a promising target for cancer therapy. It is highly upregulated in hypoxic regions and mediates pH regulation critical for tumor cell survival as well as extracellular acidification of the tumor microenvironment, which promotes tumor aggressiveness via various mechanisms, such as augmenting metastatic potential. Therefore, the aim of this study was to analyze the complex interdependency between CA IX and the tumor microenvironment in prostate tumor cells with regard to potential therapeutic implications. CA IX was upregulated by hypoxia as well as acidosis in prostate cancer cells. This induction… More >