Yahui Wu^1,2, Tiansheng Dai¹, Jingwen Qin^1,2, Jian Guo^1,2, Jitao Fan^1,2, Jun Mei^1,2, Xiaoli Li^1,2, Fang Liu^1,2,*

BIOCELL, Vol.49, No.4, pp. 701-720, 2025, DOI:10.32604/biocell.2025.061289 - 30 April 2025

Abstract Background: Dendritic cells (DCs) play a pivotal role in antigen presentation and regulating adaptive immune responses in asthma pathophysiology. However, the underlying molecular mechanisms remain incompletely understood. Methods: Bioinformatics analysis of the GSE27011 dataset identified differentially expressed genes associated with pediatric asthma. An ovalbumin (OVA)-induced asthma mouse model and an Rfx5 knockdown model were established. RFX5 expression was assessed in DCs from patients with asthma and asthmatic mouse lung tissues using qRT-PCR, Western blotting, and immunohistochemistry. The regulatory effects of regulatory factor X5 (RFX5) on histone deacetylase 2 (HDAC2), class II major histocompatibility complex transactivator… More >

HONGXIA HU^*, PING GUO, QIAN ZHAO, HAORAN LI, HUALEI LIU, CAIHONG MA

BIOCELL, Vol.45, No.4, pp. 995-1004, 2021, DOI:10.32604/biocell.2021.012851 - 22 April 2021

Abstract This study aims to detect the expression of selected circulating microRNAs (miRNA), including miRNA-451a, miRNA-486-5p and miR-10b-5p, and their prospective roles as biomarkers in patients with atherosclerosis. For this purpose, levels of miRNAs were detected by real-time quantitative polymerase chain reaction (RT-qPCR) in case (N = 30) and healthy control (N = 30) groups. Receiver operating characteristic (ROC) curve analysis was carried out to evaluate the diagnostic ability of miRNAs. The correlations of miR-451a with lipid parameters were evaluated using Pearson’s correlation coefficients. HUVEC tested by ox-LDL was used as a cellular model of atherosclerosis.… More >

Xin-sheng Cheng^*†, Shi-bo Sun^*, Feng Zhong^*, Kun He^*, Jie Zhou^*

Oncology Research, Vol.24, No.4, pp. 239-245, 2016, DOI:10.3727/096504016X14648701448011

Abstract Our aim was to study the expression of human SET domain containing protein 1A (hSETD1A) in hepatocellular carcinoma patients and its relationship with human hepatocellular carcinoma cell function. A total of 30 patients with hepatocellular carcinoma were enrolled in this study. The expression of hSETD1A was detected by real-time polymerase chain reaction (PCR) and Western blotting. The immortalized normal human liver cell line including SMMC-7721 was subjected to real-time PCR for hSETD1A mRNA. Furthermore, hSETD1A-small hairpin RNA (shRNA) was used to knock down hSETD1A expression in SMMC-7721 cells. Cell proliferation, cell apoptosis, and cell migration More >