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    ARTICLE

    Hepatocyte culture in a radial-flow bioreactor with plasma polypyrrole coated scaffolds

    Odin RAMÍREZ-FERNÁNDEZ1,*, Rafael GODÍNEZ1, Esmeralda ZUÑIGA-AGUILAR1, Luis E. GÓMEZ-QUIROZ2, María C. GUTIÉRREZ-RUIZ2, Juan MORALES3, Roberto OLAYO3

    BIOCELL, Vol.39, No.2-3, pp. 9-14, 2015, DOI:10.32604/biocell.2015.39.009

    Abstract We have designed and evaluated a radial-flow bioreactor for three-dimensional liver carcinoma cell culture on a new porous coated scaffold. We designed a culture chamber where a radial flow of culture medium was continuously delivered through it. Once this system was established, flow was simulated using flow dynamics software based on numeric methods to solve Navier-Stockes flow equations. Perfusion within cell culture scaffolds was simulated using a flow velocity of 7 mL/min and found that cell culture medium was distributed unhindered in the bioreactor chamber. Afterwards, the bioreactor was built according to the simulated design More >

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