ZULONG SHENG^*, YANRU HE, JUNYAN CAI, YUQIN JI, YUYU YAO, GENSHAN MA

BIOCELL, Vol.48, No.9, pp. 1369-1377, 2024, DOI:10.32604/biocell.2024.049905 - 04 September 2024

Abstract Background: Myocardial infarction (MI) is known worldwide for its important disabling features, including myocarditis and cardiomyocyte apoptosis. It is believed that microRNA (miRNA) has a role in the cellular processes of apoptosis and myocarditis, and miR-219a-5p has been found to suppress the inflammatory response. However, unknown is the precise mechanism by which miR-219a-5p contributes to MI. Methods: We measured the expression of miR-219a-5p and evaluated its effects on target proteins, inflammatory factors, and apoptosis in a mouse model of MI. Echocardiography was utilized to examine the MI clinical index, and triphenyl tetrazolium chloride staining was More >

Hongjun Wang^*, Shihong Zhang

Frontiers in Heat and Mass Transfer, Vol.16, pp. 1-5, 2021, DOI:10.5098/hmt.16.6

Abstract Catalytic combustion can inhibit the formation of CO and NOx, and greatly reduce the concentration of unburned alkanes. The hardness of tap water in Beijing is too high. In order to test whether the modified fly ash can reduce its hardness, clay, shale and fly ash bricks were prepared by catalytic combustion furnace. The results show that the fly ash brick has a great influence on Ca²⁺ and Mg²⁺ in water, increasing Ca²⁺ concentration by 59.96% and decreasing Mg²⁺ concentration by 41.22%. Thus modified fly ash could not reduce hardness of water but could be used as More >

Heya QIAN^1,§, Yan YAN^2,§, Zhengjie SHEN¹, Lixian XU¹, Yun ZUO¹, Tao ZHU^3,*, Yanan CHEN^1,*

BIOCELL, Vol.43, No.4, pp. 321-326, 2019, DOI:10.32604/biocell.2019.07992

Abstract The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3 in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca²⁺ were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly decreased the expressions of Rap2B protein. Additionally, knockdown of p53 More >

Fuat USLUSOY¹, Mustafa NAZIROĞLU^2,3,*

BIOCELL, Vol.43, No.4, pp. 271-283, 2019, DOI:10.32604/biocell.2019.08333

Abstract The wound is induced by several mechanical and metabolic factors. In the etiology of the wound recovery, excessive oxidative stress, calcium ion (Ca²⁺) influx, and apoptosis have important roles. Ca²⁺-permeable TRPM2 channel is activated by oxidative stress. Protective roles of Hypericum perforatum extract (HP) on the mechanical nerve injury-induced apoptosis and oxidative toxicity through regulation of TRPM2 in the experimental animals were recently reported. The potential protective roles in HP treatment were evaluated on the TRPM2-mediated cellular oxidative toxicity in the renal epithelium (MPK) cells. The cells were divided into three groups as control, wound, and wound… More >

Mingzhi Luo^1,2, Peili Yu¹, Yang Jin³, Zhili Qian¹, Yue Wang¹, Jingjing Li¹, Peng Shang^2*, Linhong Deng^1*

Molecular & Cellular Biomechanics, Vol.13, No.2, pp. 137-157, 2016, DOI:10.3970/mcb.2016.013.155

Abstract Random positioning machine (RPM) and diamagnetic levitation are two essential ground-based methods used to stimulate the effect of microgravity in space life science research. However, the force fields generated by these two methods are fundamentally different, as RPM generates a dynamic force field acting on the surface in contact with supporting substrate, whereas diamagnetic levitation generates a static force field acting on the whole body volume of the object (e.g. cell). Surprisingly, it is hardly studied whether these two fundamentally different force fields would cause different responses in mammalian cells. Thus we exposed cultured MC3T3-E1… More >