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  • Open Access

    ARTICLE

    EFFECT OF MODIFIED FLY ASH PREPARED BY CATALYTIC COMBUSTION FURNACE ON Ca2+ AND Mg2+ IN WATER

    Hongjun Wang*, Shihong Zhang

    Frontiers in Heat and Mass Transfer, Vol.16, pp. 1-5, 2021, DOI:10.5098/hmt.16.6

    Abstract Catalytic combustion can inhibit the formation of CO and NOx, and greatly reduce the concentration of unburned alkanes. The hardness of tap water in Beijing is too high. In order to test whether the modified fly ash can reduce its hardness, clay, shale and fly ash bricks were prepared by catalytic combustion furnace. The results show that the fly ash brick has a great influence on Ca2+ and Mg2+ in water, increasing Ca2+ concentration by 59.96% and decreasing Mg2+ concentration by 41.22%. Thus modified fly ash could not reduce hardness of water but could be used as More >

  • Open Access

    ARTICLE

    p53 siRNA promotes autophagy of U2OS cells through its target gene Rap2B

    Heya QIAN1,§, Yan YAN2,§, Zhengjie SHEN1, Lixian XU1, Yun ZUO1, Tao ZHU3,*, Yanan CHEN1,*

    BIOCELL, Vol.43, No.4, pp. 321-326, 2019, DOI:10.32604/biocell.2019.07992

    Abstract The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3 in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca2+ were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly decreased the expressions of Rap2B protein. Additionally, knockdown of p53 More >

  • Open Access

    ARTICLE

    An extract of Hypericum perforatum induces wound healing through inhibitions of Ca2+ mobilizations, mitochondrial oxidative stress and cell death in epithelial cells: Involvement of TRPM2 channels

    Fuat USLUSOY1, Mustafa NAZIROĞLU2,3,*

    BIOCELL, Vol.43, No.4, pp. 271-283, 2019, DOI:10.32604/biocell.2019.08333

    Abstract The wound is induced by several mechanical and metabolic factors. In the etiology of the wound recovery, excessive oxidative stress, calcium ion (Ca2+) influx, and apoptosis have important roles. Ca2+-permeable TRPM2 channel is activated by oxidative stress. Protective roles of Hypericum perforatum extract (HP) on the mechanical nerve injury-induced apoptosis and oxidative toxicity through regulation of TRPM2 in the experimental animals were recently reported. The potential protective roles in HP treatment were evaluated on the TRPM2-mediated cellular oxidative toxicity in the renal epithelium (MPK) cells. The cells were divided into three groups as control, wound, and wound… More >

  • Open Access

    ARTICLE

    Differential Responses of Cultured MC3T3-E1 Cells to Dynamic and Static Stimulated Effect of Microgravity in Cell Morphology, Cytoskeleton Structure and Ca2+ Signaling

    Mingzhi Luo1,2, Peili Yu1, Yang Jin3, Zhili Qian1, Yue Wang1, Jingjing Li1, Peng Shang2*, Linhong Deng1*

    Molecular & Cellular Biomechanics, Vol.13, No.2, pp. 137-157, 2016, DOI:10.3970/mcb.2016.013.155

    Abstract Random positioning machine (RPM) and diamagnetic levitation are two essential ground-based methods used to stimulate the effect of microgravity in space life science research. However, the force fields generated by these two methods are fundamentally different, as RPM generates a dynamic force field acting on the surface in contact with supporting substrate, whereas diamagnetic levitation generates a static force field acting on the whole body volume of the object (e.g. cell). Surprisingly, it is hardly studied whether these two fundamentally different force fields would cause different responses in mammalian cells. Thus we exposed cultured MC3T3-E1… More >

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