Katerina Ondraskova^1,2, Matous Cwik³, Ondrej Horky⁴, Jitka Berkovcova⁴, Jitka Holcakova¹, Martin Bartosik¹, Tomas Kazda⁵, Klara Mrazova^1,6, Michal Uher⁷, Igor Kiss³, Roman Hrstka^1,3,*

Oncology Research, Vol.34, No.2, 2026, DOI:10.32604/or.2025.070116 - 19 January 2026

Abstract Objectives: Cancer treatment relies heavily on accurate diagnosis and effective monitoring of the disease. These processes often involve invasive procedures, such as colonoscopy, to detect malignant tissues, followed by molecular analyses to determine relevant biomarkers. This study aimed to evaluate the clinical performance of droplet digital PCR (ddPCR) for detecting Kirsten Rat Sarcoma Viral Proto-Oncogene (KRAS), Neuroblastoma RAS Viral Oncogene Homolog (NRAS), and B-Raf Murine Sarcoma Viral Oncogene Homolog B (BRAF) mutations in circulating tumor DNA (ctDNA) from colorectal cancer patients using liquid biopsy. Methods: ctDNA was isolated from colorectal cancer (CRC) patients (n = 110) and analyzed for KRAS, BRAF,… More >

Urszula Częścik^1,2,#, Martyna Gryglas³, Arkadiusz Szterk⁴, Sylwia Flis^3,#,*

Oncology Research, Vol.34, No.2, 2026, DOI:10.32604/or.2025.067449 - 19 January 2026

Abstract Colorectal cancer (CRC) is the third most common malignancy worldwide and the second leading cause of cancer-related deaths, accounting for approximately 10% of all cancer cases. By 2050, CRC incidence is expected to rise substantially, driven by population aging and greater exposure to risk factors in developing countries. Despite advances in medicine and pharmacy, the effectiveness of available treatments remains limited, underscoring the urgent need for innovative therapeutic strategies. This review summarizes and critically evaluates currently available CRC therapies and explores new emerging directions. Particular attention is given to the role of immunotherapy, targeted therapies,… More >

Kai Gui^1,#, Tianyi Yang^1,#, Chengying Xiong¹, Yue Wang¹, Zhiqiang He¹, Wuxian Li^2,3,*, Min Tang^1,*

Oncology Research, Vol.34, No.1, 2026, DOI:10.32604/or.2025.070143 - 30 December 2025

Abstract Objectives: The mechanism by which specific tumor subsets in colorectal cancer (CRC) use alternative metabolic pathways, particularly those modulated by hypoxia and fructose, to alter the tumor microenvironment (TME) remains unclear. This study aimed to identify these malignant subpopulations and characterize their intercellular signaling networks and spatial organization through an integrative multi-omics approach. Methods: Leveraging bulk datasets, single-cell RNA sequencing, and integrative spatial transcriptomics, we developed a prognostic model based on hypoxia-and fructose metabolism-related genes (HFGs) to delineate tumor cell subpopulations and their intercellular signaling networks. Results: We identified a specific subset of stanniocalcin-2 positive (STC2+)… More > Graphic Abstract

Jiayin Peng^1,2,#, Yijun Xue^2,#, Zhiren Cai², Zhaoguan Li², Kangyan Han², Xiaoqi Lin², Yutong Li^1,*, Yumin Zhuo^1,*

Oncology Research, Vol.33, No.10, pp. 3127-3154, 2025, DOI:10.32604/or.2025.067340 - 26 September 2025

Abstract Background: Clear cell renal cell carcinoma (ccRCC) is an aggressive malignancy associated with limited treatment options and poor prognosis. Emerging studies suggest that the actin-regulating protein actin-related protein 2/3 complex subunit 1B (ARPC1B), a key regulatory protein within the actin cytoskeleton, could play a pivotal role in ccRCC progression. The current study aimed to uncover the biological functions of ARPC1B and the molecular mechanisms driving its effects in ccRCC. Methods: ARPC1B expression and prognostic implications were analyzed using data sourced from the Gene Expression Profiling Interactive Analysis (GEPIA) platform, immunohistochemical (IHC) staining on 150 tumor… More >

Yuhang Jiang^#, Yijun Xu^#, Qi Zhu, Yingxia Wu, Zhe Wang, Shuang He, Shiyong Yu^*, Honggang Xiang^*

Oncology Research, Vol.33, No.9, pp. 2379-2398, 2025, DOI:10.32604/or.2025.063501 - 28 August 2025

Abstract Background: Colorectal cancer (CRC) is common and deadly, often leading to metastasis, challenging treatment, and poor outcomes. Understanding its molecular basis is crucial for developing effective therapies. Aims: This study aimed to investigate the role of Myosin Heavy Chain 11 (MYH11) in CRC progression, especially its effects on epithelial-mesenchymal transition (EMT) and cell behavior, and to explore its potential regulation by the EMT transcription factor zinc finger E-box binding homeobox 1 (ZEB1). Methods: Differential expression analysis was performed in the GSE123390 and TCGA-READ datasets, and 317 intersection genes were identified. The hub gene MYH11 was identified… More >

Jize Zhang¹, T. P. C. Klaver², Songge Yang¹, Brajendra Mishra¹, Yu Zhong^1,*

CMC-Computers, Materials & Continua, Vol.84, No.3, pp. 4299-4311, 2025, DOI:10.32604/cmc.2025.067793 - 30 July 2025

Abstract Designing high-performance high-entropy alloys (HEAs) with transformation-induced plasticity (TRIP) or twinning-induced plasticity (TWIP) effects requires precise control over stacking fault energy (SFE) and phase stability. However, the vast complexity of multicomponent systems poses a major challenge for identifying promising candidates through conventional experimental or computational methods. A high-throughput CALPHAD framework is developed to identify compositions with potential TWIP/TRIP behaviors in the Cr-Co-Ni and Cr-Co-Ni-Fe systems through systematic screening of stacking fault energy (SFE), FCC phase stability, and FCC-to-HCP transition temperatures (T₀). The approach combines TC-Python automation with parallel Gibbs energy calculations across hundreds of thousands of… More >

Chuncheng Liu¹, Xiaohan Liu¹, Ziqi Li¹, Yanruoxue Wei¹, Bangdong Liu², Peng Zhu², Yukun Liu^1,2,*, Ran Zhao^1,2,*

Oncology Research, Vol.33, No.8, pp. 2085-2105, 2025, DOI:10.32604/or.2025.065739 - 18 July 2025

Abstract Background: VPS37A (VPS37A subunit of ESCRT-I), a component of the ESCRT-I (endosomal sorting complex required for transport I) complex, mediates vesicular trafficking through sorting endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Although accumulating evidence indicates that VPS37A deficiency occurs in numerous malignancies and exerts tumor-suppressive effects during cancer progression, its functional significance in colorectal cancer (CRC) pathogenesis remains poorly characterized. Therefore, this study aims to further investigate the functional and molecular mechanisms by which VPS37A downregulation contributes to malignant biological phenotypes in CRC, with a specific focus on how its dysregulation affects cell death pathways.… More > Graphic Abstract

Fang Xu^1,2,#, Wan Li^1,#, Xiang-Jin Zheng^1,2, Yue Hao^1,2, Yi-Hui Yang^1,2, Hong Yang^1,2, Sen Zhang^1,2, Wan-Xin Cao^1,2, Xiao-Xue Li^1,2, Xu Zhang^1,2, Guan-Hua Du^1,2, Teng-Fei Ji^1,*, Jin-Hua Wang^1,2,*

Oncology Research, Vol.33, No.8, pp. 1969-1989, 2025, DOI:10.32604/or.2025.062386 - 18 July 2025

Abstract Objectives: Colorectal cancer (CRC) is one of the most common cancers all over the world. The progression of CRC is associated with inflammation and disruptions in intestinal flora. 3-O-Acetyl-11-keto-β-boswellic acid (AKBA) has been noted for its potent anti-inflammatory properties. However, the effect of AKBA on colon cancer caused by inflammation and its mechanism are not unclear. The study is to explore the effect of AKBA on CRC and its mechanism. Materials and Methods: Cell proliferation, (5-ethynyl-2^′-deoxyuridine, EdU)-DNA synthesis assay and colony formation were used to assess the effect of AKBA on the proliferation of CRC cells.… More >

Guo-Zhi Xu^1,2, Han-Yang Guan¹, Yan-Guan Guo¹, Yi-Ran Zhang¹, Jing-Hua Pan¹, Simin Luo³, Hui Ding¹, Yunlong Pan^1,*, Qi Yao^4,*

Oncology Research, Vol.33, No.8, pp. 1947-1967, 2025, DOI:10.32604/or.2025.060440 - 18 July 2025

Abstract Background: Tumor angiogenesis is related to solid tumor occurrence. Ubiquitin-specific peptidase 13 (USP13) is a deubiquitinating enzyme with a pivotal effect on tumor proliferation, metastasis, and tumorigenesis. Nonetheless, its effect on colorectal cancer (CRC) angiogenesis remains poorly understood. Methods: Human umbilical vein endothelial cells (HUVECs) and CRC cells were cultivated, followed by USP13 knockdown/overexpression using shRNA lentiviral vectors or plasmids. Conditioned media (CM) from treated CRC cells were collected to assess HUVEC migration, invasion, and tube formation. Phosphatase and tensin homolog (PTEN) overexpression and recombinant vascular endothelial growth factor A (VEGFA) rescue experiments were performed.… More >

RAQUEL GÓMEZ-MOLINA^1,*, RAQUEL MARTÍNEZ^2,3,4, MIGUEL SUÁREZ^2,3,4,*, ANA PEÑA-CABIA¹, MARíA CONCEPCIóN CALDERÓN¹, JORGE MATEO^3,4

Oncology Research, Vol.33, No.7, pp. 1531-1545, 2025, DOI:10.32604/or.2025.063951 - 26 June 2025

Abstract Lynch syndrome (LS), also known as hereditary non-polyposis colorectal cancer (HNPCC), is an inherited condition associated with a higher risk of colorectal cancer (CRC) and other cancers. It is caused by germline mutations in DNA mismatch repair (MMR) genes, including MLH1, MSH2, MSH6 and PMS2. These mutations lead to microsatellite instability (MSI) and defective DNA repair mechanisms, resulting in increased cancer risk. Early detection of LS is crucial for effective management and cancer prevention. Endoscopic surveillance, particularly regular colonoscopy, is recommended for individuals with LS to detect CRC at early stages. Additionally, universal screening of CRC for More > Graphic Abstract