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    Induction of Apoptosis and Autophagy Using Ectopic DSCR1 Expression in Breast Cancer Cells

    Zahra Niki Boroujeni1, Atefeh Shirkav1, Seyed Ahmad Aleyasin1,*

    Molecular & Cellular Biomechanics, Vol.15, No.4, pp. 215-227, 2018, DOI:10.32604/mcb.2018.01813

    Abstract Down syndrome critical region 1 gene (DSCR1) is an anti-angiogenesis gene that inhibits the growth of tumor cells. In this study, the role of autophagy and apoptosis in DSCR1-induced cytotoxicity were investigated in MDA-MB-468 breast cancer cells. Lentivirus vector harboring DSCR1 (LV-DSCR1+) was constructed in HEK 293 cells and the optimal dosage of lentivirus vector for infection was determined by the MTT assay. After infection of cells using LV-DSCR1+, acridine orange and ethidium bromide staining was performed to investigation of apoptosis and autophagy. Expression of DSCR1 and marker genes for angiogenesis (VEGF), apoptosis (Bax and Bcl2) and autophagy (LC3 and… More >

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