BINWU BAO, JINPENG WANG, YANXIA LI, FEN FENG, ZHONGXIANG JI, ZHUOMA LUORENG^*, XINGPING WANG^*

BIOCELL, Vol.47, No.7, pp. 1509-1518, 2023, DOI:10.32604/biocell.2023.028646 - 21 June 2023

Abstract Bovine oocytes are one of the indispensable cells in cattle reproduction and have become a research hot spot in cattle reproduction in recent years. The maturation process of oocytes is mainly regulated by enzymes, hormones, cytokines, and other molecules. The factors affecting cattle oocyte maturation have been previously studied to clarify the molecular mechanisms of cattle oocyte maturation. In this review article, phospholipid protein-3-kinase/protein kinase B, mitogen-activated protein kinase/extracellular signal-regulated kinase, Janus kinase/signal transducer and activator of transcription, epidermal growth factor receptor/extracellular signal-regulated kinase, and other signaling pathways related to oocyte maturation are discussed. In More >

ANA CECILIA LIAUDAT, FRANCISCA EBEL, BIANCA ANA OPIZZO, MARINA AURORA GONZALEZ, DAMIÁN BLOIS, PABLO BOSCH^#, NANCY RODRIGUEZ^#,*

BIOCELL, Vol.47, No.5, pp. 1135-1143, 2023, DOI:10.32604/biocell.2023.027628 - 10 April 2023

Abstract Background: Capacitation is a set of physiological changes sperms undergo to acquire fertilizing capacity. In vivo, this process is directly associated with high calcium levels in sperm cytoplasm. Calcitriol, the vitamin D hypercalcemic metabolite, is related to human sperm motility, capacitation, and acrosome reaction. This work aimed to study the effect of calcitriol on bull sperm quality parameters and capacitation. Methods: One million freeze-thawed spermatozoa were obtained from different bulls and treated with 20 nM of calcitriol for 30 min. Untreated cells (negative control) and treated ones with calcitriol or heparin (100 µg/mL, positive capacitation control) were… More >

ABDELAZIZ ADAM IDRISS ARBAB^1,3,#, CHUNQING YIN^4,#, XUBIN LU¹, YAN LIANG¹, ISMAIL MOHAMED ABDALLA¹, AMER ADAM IDRIS³, TIANLE XU^1,2, YONGJIANG MAO¹, ZHANGPING YANG^1,2,*

BIOCELL, Vol.46, No.11, pp. 2443-2454, 2022, DOI:10.32604/biocell.2022.020865 - 07 July 2022

Abstract Mastitis is a common inflammatory cow mammary infection; that causes significant economic loss in dairy industry. Given the interesting connection between metformin’s anti-inflammatory function and mastitis model induced by LTA in pbMECs, our objective was to prove that metformin was beneficial in suppressing proinflammatory response induced by LTA through modulation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways and activation of peroxisome proliferator-activated receptor-γ (PPARγ) in pbMECs. The proliferation of cells and mRNA expression were measured using EdU assay and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Immunoblotting and immunofluorescence analysis… More >

KANG ZHAN^#, MAOCHENG JIANG^#, TIANYU YANG, ZIXUAN HU, GUOQI ZHAO^*

BIOCELL, Vol.46, No.7, pp. 1697-1704, 2022, DOI:10.32604/biocell.2022.019068 - 17 March 2022

Abstract Short-chain fatty acids (SCFAs) butyrate promote the postnatal rumen epithelial development and maturation in ruminants. However, molecular mechanisms of effects of butyrate on the bovine rumen epithelial cells (BRECs) proliferation remain elusive. Therefore, purpose of this study was to investigate the effects of butyrate on the expression of genes and proteins at G0/G1 and S phase of BRECs cycle. Our results showed that BRECs treated with butyrate inhibited (P < 0.05) the proliferation of BRECs, relatively to control. Flow cytometric assays revealed that butyrate triggers the BRECs cycle arrest at the G0/G1 phase. qRT-PCR analyses of… More >

JINGPENG ZHOU^1,2,#, XIAOGUANG JI^3,#, YUHAO WANG^1,2, XIAOLONG WANG^1,2, YONGJIANG MAO^1,2,*, ZHANGPING YANG^1,2,*

BIOCELL, Vol.45, No.4, pp. 1033-1044, 2021, DOI:10.32604/biocell.2021.015586 - 22 April 2021

Abstract Cow mastitis is the most common disease that affects the dairy farming industry and causes serious harm to dairy cows and humans, and Staphylococcus (S.) aureus is one of the main pathogens that cause mastitis in dairy cows. In this study, a mastitis model was established through the infection of bovine mammary epithelial cells (BMECs) with S. aureus (bacterial concentration of 1 × 10⁹/mL), and these cells and a blank group (untreated) were analyzed by flow cytometry (10000 cells, 200 cells collected per second), hematoxylin and eosin (H&E) staining and immunohistochemistry. In addition, the lncRNAs (long non-coding RNAs)… More >

SERGIO GASTÓN CASPE¹, JOSÉ LUIS KONRAD², DADIN PRANDO MOORE³, JUAN MANUEL SALA¹, LILIAN LISCHINSKY³, CARLOS MANUEL CAMPERO³, CLAUDIO GUSTAVO BARBEITO^4,*

BIOCELL, Vol.45, No.2, pp. 331-337, 2021, DOI:10.32604/biocell.2021.010701 - 19 February 2021

Abstract Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples… More >

MINGHAO LI¹, YOULI QIU², WENHUI ZHANG¹, RUIHAO SUN¹, MEIJIN DU¹, LUZE YANG³, YU LI^1,*

BIOCELL, Vol.44, No.2, pp. 247-255, 2020, DOI:10.32604/biocell.2020.08835 - 27 May 2020

Abstract While phthalate acid esters (PAEs) cannot fluoresce alone, they can be detected by fluorescence spectroscopy after chelation with bovine serum albumin (BSA). In this study, the types of amino acid residues at the active site of PAEs chelated with BSA were determined using molecular docking technology. A modification scheme of BSA with higher detection sensitivity fluorescence spectroscopy for PAEs was proposed based on the docking results and constructed for a novel BSA structure with a higher detection sensitivity of fluorescence spectroscopy using a homologous modeling method. Density functional theory (DFT) was employed to explore the… More >

Longjia Li^1,2, Tianbao Qian^2,3, Fei Hang^{1, 2, *}

Molecular & Cellular Biomechanics, Vol.14, No.4, pp. 221-234, 2017, DOI:10.3970/mcb.2017.014.221

Abstract Bone is an important natural hierarchical biomaterial which supports human body and protect organs. Its mechanical property has been researched extensively. In this experimental work, 3D microscope and scanning electron microscope (SEM) were used to research crack expansion on bovine femur cortical bone transverse section. Softwares such as image J and Photoshop were used in image and data analyses. Our results suggested that the interface energy of extending through osteons is smaller than the interface energy of extending along cement lines. Cracks are more likely to extend through osteons. Further investigations are needed to reduce More >

ROMINA J. BEVACQUA, RAFAEL FERNANDEZ-MARTIN, DANIEL F. SALAMONE

BIOCELL, Vol.35, No.1, pp. 1-7, 2011, DOI:10.32604/biocell.2011.35.001

Abstract Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid… More >

A. LÓPEZ-HERRERA^1*, J. RUIZ-SÁENZ², Y.P. GÓEZ³, W. ZAPATA³, P.A. VELILLA³, A.E. ARANGO³, S. URCUQUI-INCHIMA³

BIOCELL, Vol.33, No.2, pp. 121-132, 2009, DOI:10.32604/biocell.2009.33.121

Abstract To determine whether fibroblasts from Blanco Orejinegro cattle, exhibit any level of resistance to infection against vesicular stomatitis virus (VSV) serotypes Indiana (VSV-I) or New Jersey (VSVNJ), 30 fibroblast cultures were phenotyped to evaluate their resistance/susceptibility. Thirty three % of Blanco Orejinegro fibroblast cultures were classified as very resistant, 50% as resistant, and 17% as susceptible to VSV-I infection, whereas 20% were classified as very resistant, 50% as resistant and 30% as susceptible to VSV-NJ infection. Therefore, there appears to be a large variation in phenotypic polymorphism among the fibroblasts to infection by VSV. To More >