Olga S. Tsiftsoglou^1,*, Vladimir Mihailovic², Nikola Sreckovic², Jelena S. Katanic Stankovic³, Kyriakos Michail Dimitriadis¹, Michalis K. Stefanakis⁴, Diamanto Lazari¹

Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.075566 - 30 January 2026

Abstract Achillea species are known for their healing properties since ancient times. There is extensive literature on their pharmacological action due to their bioactive compounds. The present study aimed to investigate the antioxidant and antimicrobial effects of hydroalcoholic extracts from the inflorescences and leaves of the species Achillea crithmifolia Waldst. and Kit., A. grandifolia Friv. and A. millefolium L. The phytochemical profiles of all extracts were evaluated both by NMR spectroscopy and LC-MS analysis, and the results were consistent with the spectrophotometrically determined total phenolic (TP: 125.42–191.98 mg/g) and total flavonoid (TF: 47.34–180.02 mg/g) contents. All the extracts were tested More >

Luksamee Vittaya^1,*, Chakhriya Chalad¹, Sittichoke Janyong², Nararak Leesakul³

Phyton-International Journal of Experimental Botany, Vol.94, No.12, pp. 4091-4115, 2025, DOI:10.32604/phyton.2025.074891 - 29 December 2025

Abstract Calophyllum inophyllum is a tropical plant that could have useful medicinal properties for pharmaceutical and cosmetic applications. The present study extracted the flower, fruit, leaf, twig, and bark of the plant by maceration in different organic solvents. The correlation between bioactive compounds and their biological activities was investigated, with emphasis on their therapeutic relevance through in silico pharmacokinetic predictions using SwissADME. Qualitative and quantitative analyses were conducted to determine the total phenolic, flavonoid, and saponin contents of the extracts. Spectral analysis of the extracts revealed –OH, C=O, C=C, and C–H functional groups. The antioxidant activity of the… More > Graphic Abstract

Yuhan Xie¹, Shaden A. M. Khalifa², Hesham R. El-Seedi³, Paolo Coghi^1,4,*

Oncology Research, Vol.33, No.10, pp. 2673-2697, 2025, DOI:10.32604/or.2025.067666 - 26 September 2025

Abstract Podophyllotoxin is a well-studied natural product. Because of its unique structure and ability to inhibit cancer cells, it has been changed in different ways to find out its pharmacological properties. This paper discusses the common chemical modifications of podophyllotoxin molecules, including the C-4 and E-4 site replacements. Furthermore, its common inhibitory effects on cancer cells and antiparasitic activities, among others, are outlined by the connection between conformational changes and pharmacological activities. Importantly, Podophyllotoxin can effectively overcome the phenomenon of multidrug resistance through a dual-targeting mechanism, including inhibition of microtubule protein synthesis and topoisomerase II activity, More > Graphic Abstract

Jin Liu^1,2, Xiu Zhang¹, Xiao Hu², Gaofeng Yuan², Kai Chen^1,*

BIOCELL, Vol.49, No.4, pp. 665-680, 2025, DOI:10.32604/biocell.2025.063296 - 30 April 2025

Abstract Objective: This study examines the significance and functions of CLDN9 in gastric cancer (GC), intending to identify novel targets for diagnosis and treatment. Methods: CLDN9 expression in GC tissues and cell lines was investigated in TCGA data, with analysis with Western blotting, qRT-PCR, and immunohistochemical analyses. Correlations between clinicopathological characteristics, progression-free survival (PFS), and overall survival (OS) were assessed with Cox regression. The effect of CLDN9 knockdown/overexpression on tumorigenic functions (proliferation, migration, and invasion) was assessed using CCK-8, colony formation, and Transwell assays. Tumor-bearing assays were performed to verify the impact of CLDN9 knockdown on… More >

Longgang Wang^2,3,#, Xiangxun Zhuansun^2,3,#, Yao Li^2,3, Qili Yao^2,3, Qi Liu^2,3,*, Huijing Lin^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 441-454, 2025, DOI:10.32604/phyton.2025.061720 - 06 March 2025

Abstract Fructus Ligustri Lucidi (FLL) refers to the dried mature fruit of Ligustrum lucidum Ait., a species from the Oleaceae family, widely distributed across East Asia and India. This study aimed to optimize the extraction process for Fructus Ligustri Lucidi essential oil (FLLO) to develop an efficient and practical extraction method. Additionally, the chemical composition of FLLO was analyzed, and its antioxidant, antimicrobial, and cytotoxic activities were evaluated. FLLO was extracted using supercritical CO₂ extraction, and response surface methodology was applied to optimize the extraction parameters: pressure of 16 MPa, temperature of 40°C, and extraction time of 40… More > Graphic Abstract

Oliver Tusevski^1,*, Marija Todorovska¹, Jasmina Petreska Stanoeva², Marina Stefova², Sonja Gadzovska Simic¹

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 921-941, 2023, DOI:10.32604/phyton.2023.024995 - 29 November 2022

Abstract Three Hypericum perforatum hairy root lines (HR B, HR F and HR H) along with non-transformed roots were analyzed for phenolic compounds composition and in vitro enzyme inhibitory properties. In silico molecular modeling was performed to predict the interactions of the most representative phenolic compounds in HR clones with enzymes related to depression, neurodegeneration and diabetes. Chromatographic analyses revealed that HR clones represent an efficient source of quinic acid and hydroxybenzoic acids, epicatechin and procyanidin derivatives, quercetin and kaempferol glycosides, as well numerous xanthones. In vitro antidepressant activity of HR extracts through monoamine oxidase A (MAO-A) inhibition was attributed… More >

Ayda Khadhri¹, Chedia Aouadhi², Eric Masson³, Antonio Pizzi^4,5,*

Journal of Renewable Materials, Vol.10, No.6, pp. 1607-1621, 2022, DOI:10.32604/jrm.2022.018950 - 20 January 2022

Abstract Salvia microphylla and Teucrium polium are medicinal and aromatic plants with ethnobotanical uses. The present study was conducted to investigate the chemical composition of Salvia microphylla and Teucrium polium essential oils, the secondary metabolites, and the biological activities of their infusion and methanolic (MeOH) extracts. Essential oils were extracted by hydrodistillation from shoots Salvia microphylla and Teucrium polium. Phenolic content, antioxidant and antimicrobial activities were determined. Gas chromatography-mass spectrometry (GC-MS) results showed the presence of significant qualitative and quantitative variations for the composition of the both essential oils (EO). Salvia microphylla EO were most complex and present 70 compounds with the major… More >

ELIF ANT BURSALI^1,*, DILER ABACI¹, MURAT KIZIL², MURUVVET YURDAKOC¹

Journal of Polymer Materials, Vol.38, No.3-4, pp. 231-245, 2021, DOI:10.32381/JPM.2021.38.3-4.5

Abstract Genipin cross-linked/boron doped starch/polyvinily alcohol (PVA) based hydrogel (SH-GNP-B) was synthesized as a new material having antimicrobial and antioxidant activity. The prepared hydrogel was characterized by X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Thermal Gravimetric Analysis (TGA) and Scanning Electron Microscope (SEM) methods and evaluated for in vitro antimicrobial activities against selected organisms by disc diffusion tests. The antioxidant activity of the prepared hydrogels was evaluated using 2,2-diphenyl-1- picrylhydrazyl radical scavenging assays. Swelling behavior of the hydrogel was also investigated. The synthesized hydrogel was thermally stable and showed pH independent swelling tendency. SH-GNP-B hydrogel More >