Buentello Volante1 B, F Díaz de León-Sánchez1, F Rivera-Cabrera1, R Aguilar Caballero2, M Ponce-Valadez1, E Sánchez de Jiménez2, LJ Pérez-F lores1
Phyton-International Journal of Experimental Botany, Vol.79, pp. 101-108, 2010, DOI:10.32604/phyton.2010.79.101
Abstract Auxin and insulin promote Zea mays embryo growth, induce S6 ribosomal protein (S6rp) phosphorylation, and promote specific protein synthesis. The objective of this research was to test a possible cross-talk between insulin and auxin transduction pathways in Z. mays coleoptiles, typical auxin target tissues. Auxin and insulin produced differential quantitative and qualitative stimulation of cytoplasmic and ribosomal protein phosphorylation, and specific patterns of de novo synthesized cytoplasmic proteins. In addition, insulin induced S6rp phosphorylation was strongly inhibited by rapamycin, indicating target of rapamycin (TOR) kinase participation; auxin-induced S6rp phosphorylation was insensitive to this inhibitor. Phosphatidic acid (PA), a More >