Yuan Tian, Lulu Zhi, Ping Li^*, Xiangyang Hu^*

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1101-1116, 2024, DOI:10.32604/phyton.2024.050220 - 27 June 2024

Abstract Seed viability is an essential feature for genetic resource conservation as well as sustainable crop production. Long-term storage induces seed viability deterioration or seed aging, accompanied by the accumulation of toxic reactive oxygen species (ROS) to suppress seed germination. Controlled deterioration treatment (CDT) is a general approach for mimicking seed aging. The transcription factor ANAC089 was previously reported to modulate seed primary germination. In this study, we evaluated the ability of ANAC089 to control seed viability during aging. Compared with that in the wild-type line, the mutation of ANAC089 significantly increased HO, thereby reducing seed viability after… More >

Fenglan Wang^#, Zhenyuan Cai^#, Zhimei Li, Shilong Zhang, Honghui Luo, Qing Wu, Hanhan Xia, Yanhong Guo^*

Phyton-International Journal of Experimental Botany, Vol.93, No.4, pp. 819-830, 2024, DOI:10.32604/phyton.2024.049150 - 29 April 2024

Abstract FLOWERING LOCUS T (FT) is an important factor for integrating flowering signals through the photoperiod pathway, which significantly promotes flowering. Most chrysanthemum varieties are short-day plants, sensitive to the photoperiod, which limits their ornamental and annual production supply. In this study, we aim to analyze the nutrients and flowering-related genes of chrysanthemums with different photoperiod types and to clone and verify the function of the flowering-related gene CmFT. We found that the formation of floral buds requires the accumulation of starch while consuming soluble sugars and the expression patterns of flowering-related genes GIGANTEA (GI), CONSTANS (CO), and FT in C.morifolium… More >

Lei Liang, Xiangyang Hu^*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 517-531, 2024, DOI:10.32604/phyton.2024.049277 - 28 March 2024

Abstract With the rapid development of modern molecular biology and bioinformatics, many studies have proved that transcription factors play an important role in regulating the growth and development of plants. SPATULA (SPT) belongs to the bHLH transcription family and participates in many processes of regulating plant growth and development. This review systemically summarizes the multiple roles of SPT in plant growth, development, and stress response, including seed germination, flowering, leaf size, carpel development, and root elongation, which is helpful for us to better understand the functions of SPT. More >

Mingda Yin^1,#, Rui Luo^1,#, Tana Liang¹, Qi Wen¹, Xiaotian Liang¹, Yanpeng Wen¹, Xuemei Hu¹, Zhiyan Wang¹, Chang Gao¹, Wenjing Ren¹, Fenglan Huang^1,2,3,4,5,*

Phyton-International Journal of Experimental Botany, Vol.93, No.1, pp. 97-117, 2024, DOI:10.32604/phyton.2023.031228 - 26 January 2024

Abstract Castor is one of the top 10 oil crops in the world and has extremely valuable uses. Castor inflorescences directly affect yield, so the study of inflorescence development is very important in increasing castor yield. Our previous studies have shown that the PIP5K gene family (PIP5Ks) is associated with inflorescence development. In this study, to determine the function of each PIP5K gene in castor, a female Lm-type castor line, aLmAB2, was used to determine the relative expression levels of the PIP5Ks in castor inflorescences. Six PIP5K genes were heterologously overexpressed in Arabidopsis thaliana, the relative expression of each gene… More >

Yueying Liu^#, Yuqing Wang^#, Lijiao Gu^*

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 2937-2946, 2023, DOI:10.32604/phyton.2023.043306 - 24 October 2023

Abstract WRKY transcription factors play important roles in plant growth, development, and stress responses. Our previous research has shown that the GhWRKY91 gene can delay age-, abscisic acid (ABA)-, and drought-induced leaf senescence when overexpressed in transgenic Arabidopsis plants. To explore in more depth the biological functions of the GhWRKY91 gene, we further observed the root growth of overexpressing transgenic Arabidopsis thaliana under ABA and drought treatment. In this study, we transplanted the germinated seeds of wild-type (WT) and three transgenic lines (OE-12, OE-13 and OE-20) to 1/2 MS solid medium containing ABA and different concentrations of mannitol (simulated… More >

Yanpeng Wen^1,#, Rui Luo^1,#, Lili Li¹, Xiaotian Liang¹, Xuemei Hu¹, Ruxin Li¹, Zhiyan Wang¹, Fenglan Huang^1,2,3,4,5,*

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3091-3112, 2023, DOI:10.32604/phyton.2023.030960 - 24 October 2023

Abstract Castor (Ricinus communis L.) is one of the top 10 oil crops in the world, and inflorescence is a trait that directly affects its yield. Phospholipase C (PLCs) is involved in many plant activities and metabolic processes. To study the functions of PLC family genes in the regulation of the inflorescence development of the female line of Lm-type castor aLmAB2, we determined the expression levels of six PLC family genes of three types of inflorescences of aLmAB2 (isofemale line, female line, bisexual line) at different developmental stages. The results showed that the 6 genes of the castor PLC… More >

Lulu Zhi, Xiangyang Hu^*

Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2825-2840, 2023, DOI:10.32604/phyton.2023.030912 - 15 September 2023

Abstract Plant regeneration is a self-repair of the plant body in response to adverse conditions or damaged structures, and root regeneration allows the plant body to better adapt to its environment by supplementing the roots’ structure. Previous research has shown that adventitious roots can be made to occur from scratch in two ways. Studies that simulate adventitious root regeneration through natural conditions allow the regeneration process to be broadly divided into three stages: the perception of early signals, the massive accumulation of auxin, and the transformation of cell fate. The strength of regeneration, in turn, is More >

Zijian Cao^#, Guoxia Xue^#, Lingfeng Hu, Haoxian Qu, Shuang Liang, Jisen Shi, Jinhui Chen^*, Zhaodong Hao^*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2383-2397, 2023, DOI:10.32604/phyton.2023.029845 - 25 June 2023

Abstract The auxin polar transporter, PIN-FORMED 2 (PIN2) plays an important role in root development. However, it remains unclear whether PIN2 genes form two Liriodendron species, L. chinense (LcPIN2) and L. tulipifera (LtPIN2), are both involved in root development and whether and to what extent these two genes diverge in function. Here, we cloned and overexpressed LcPIN2 and LtPIN2 in Arabidopsis thaliana wild-type (WT) and Atpin2 mutant. Phylogenetic and sequence analysis showed a small degree of differentiation between these two Liriodendron PIN2 genes. Tissue-specific gene expression analysis indicated that both Liriodendron PIN2 genes were highly expressed in roots, implying a potential role in root… More >

Xiaokui Huang¹, Yingbin Xue¹, Aaqil Khan¹, Hanqiao Hu¹, Naijie Feng^1,2,*, Dianfeng Zheng^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2369-2382, 2023, DOI:10.32604/phyton.2023.027457 - 25 June 2023

Abstract For the purpose of functional validation, the mung bean (Vigna radiata) VrPR gene was cloned and overexpressed in Arabidopsis thaliana. The findings revealed that the ORF of VrPR contained 1200 bp, in which 399 amino acids were encoded. Bioinformatics analysis showed that the VrPR protein belonged to the NADB Rossmann superfamily, which was one of the non-transmembrane hydrophilic proteins. VrPR was assumed to have 44 amino acid phosphorylation sites and be contained in chloroplasts. The VrPR secondary structure comprised of random coil, α helix, β angle, and extended chain, all of which were quite compatible with the anticipated tertiary structure. More >

Iva Šutevski^1,#, Klara Krmpotić^1,#, Sandra Vitko¹, Nataša Bauer¹, Eva Fancev², Mario Cifrek², Željka Vidaković-Cifrek^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 901-920, 2023, DOI:10.32604/phyton.2023.025165 - 29 November 2022

Abstract Mechanical stimulation of plants can be caused by various abiotic and biotic environmental factors. Apart from the negative consequences, it can also cause positive changes, such as acclimatization of plants to stress conditions. Therefore, it is necessary to study the physiological and biochemical mechanisms underlying the response of plants to mechanical stimulation. Our aim was to evaluate the response of model plant Arabidopsis thaliana to a moderate force of 5 N (newton) for 20 s, which could be compared with the pressure caused by animal movement and weather conditions such as heavy rain. Mechanically stimulated leaves… More >