Qun Zhao, Bi-Bo Tan, Yong Li, Li-Qiao Fan, Pei-Gang Yang, Yuan Tian
Oncology Research, Vol.23, No.3, pp. 129-136, 2015, DOI:10.3727/096504015X14500513118029
Abstract In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance
through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected
into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate
of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied
to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related
genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OCUM-2MD3
and gastric epithelial cells. After HIF-1α-siRNA More >
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