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  • Open Access

    ARTICLE

    Taguchi Design of the Experimental approach to Increase the Biomass and Cell Wall Chitosan Contents of Zygomycetous Dimorphic Fungus Benjaminiella Poitrasii

    S.R. MANE1,2, E. K. PATHAN3, G. PATIL4, S. G. TUPE5, V. GHORMADE4, B. P. CHAUDHARI1,2, M.V. DESHPANDE5,*

    Journal of Polymer Materials, Vol.38, No.3-4, pp. 219-230, 2021, DOI:10.32381/JPM.2021.38.3-4.4

    Abstract A dimorphic fungus Benjaminiella poitrasii contains high chitin/chitosan (35% of the cell wall) in the mycelial (M) form than its yeast (Y) form (20% of the cell wall). However, the relative proportion of chitosan is more in yeast form cells (chitosan: chitin ratio, 6:1) than mycelial cells (chitosan: chitin ratio, 3:1). Using the Taguchi design of experimental (DOE) approach, interactions among eight different parameters showed that carbon source (starch, 10 g/L), incubation time (48 h), inoculum (M and Y mixed 10%), yeast extract (6 g/L) and peptone (10 g/L), were optimum for maximum biomass production. Under these conditions, the chitosan… More >

  • Open Access

    ARTICLE

    Evaluation of the Antibacterial and Antifungal Capacity of Nanoemulsions Loaded with Synthetic Chalcone Derivatives Di-Benzyl Cinnamaldehyde and Benzyl 4-Aminochalcone

    Flavia Oliveira Monteiro da Silva Abreu1,2,*, Taysse Holanda1, Joice Farias do Nascimento1, Henety Nascimento Pinheiro1, Rachel Menezes Castelo1, Hélcio Silva dos Santos3, Thais Benincá4, Patrícia da Silva Malheiros4, Júlio César Sousa Prado5, Raquel de Oliveira Fontenelle5, Maria Madalena de Camargo Forte2

    Journal of Renewable Materials, Vol.12, No.2, pp. 285-304, 2024, DOI:10.32604/jrm.2023.043919

    Abstract With the increase in antimicrobial resistance, it has become necessary to explore alternative approaches for combating and preventing diseases. DB-cinnamaldehyde (CNM) and Benzyl4-amino (B4AM) are bioactive compounds derived from chalcones but with restricted solubility in aqueous media. Nanoemulsions can enhance the solubility of compounds and can be a promising alternative in the development of novel antimicrobials, with reduced side effects and prolonged release. The objective of this study was to evaluate the stability of oil-in-water nanoemulsions loaded with two distinct types of chalcones at two different dosages, to propose a stable formulation with antimicrobial properties. Results showed that nanoemulsions presented… More > Graphic Abstract

    Evaluation of the Antibacterial and Antifungal Capacity of Nanoemulsions Loaded with Synthetic Chalcone Derivatives Di-Benzyl Cinnamaldehyde and Benzyl 4-Aminochalcone

  • Open Access

    ARTICLE

    Development of Molecular Marker Linked with Cercospora Leaf Spot (CLS) Disease Resistance in Vigna radiata, Cloning, and Expression for Evaluating Antifungal Activity against Cercospora canescens

    Maria Babar1, Siddra Ijaz1,*, Imran Ul Haq2, Muhammad Sarwar Khan1

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1289-1300, 2023, DOI:10.32604/phyton.2023.026469

    Abstract We developed a molecular marker for MAS of mungbean resistant varieties against CLS from the consensus sequence (MB-CLsRG) of identified RGAs (MB-ClsRCaG1 and MB-ClsRCaG2). The MB-CLsRG sequence-specific primer pair was used to screen Cercospora leaf spot (CLS) resistant varieties of mungbean in genomic analysis that showed congruency with phenotypic screening. Validation of molecular marker linkage with CLS resistance was performed using rtPCR in transcriptomic analysis. The sequenced PCR products showed 100% homology with MB-CLsRG sequence and putative disease resistance proteins that confirmed the linkage of molecular marker with CLS resistance in mungbean. The antifungal potential of MB-CLsRG gene encoding protein… More >

  • Open Access

    ARTICLE

    Antifungal Potential of Beauveria bassiana on Solanum lycopersicum L. Infected with Fusarium oxysporum f. sp. lycopersici

    Henry López-López1, Nancy Ruiz-Lau2, Rocío Meza-Gordillo1, Víctor Manuel Ruiz-Valdiviezo1, Joseph Galthier Robledo-Luchetti1, Carlos Alberto Lecona-Guzmán1, Juan José Villalobos-Maldonado1, Luc Dendooven3, Joaquín Adolfo Montes-Molina1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1235-1255, 2023, DOI:10.32604/phyton.2023.025716

    Abstract The objective of this work was to evaluate the effect of Beauveria bassiana (Bb 1205) on controlling Fusarium oxysporum f. sp. lycopersici (Fol 17108) in tomato plants in greenhouse conditions. Inoculation of Bb 1205 was the most promising among the agronomic variables and expression of the activity of the enzymes β-1,3-glucanases and chitinases. Inoculation of Bb 1205 occurred at a concentration of 1 × 108 conidia·mL−1, which was administered onto the leaves, directly into the soil and via injection. Infection with Fol 17108 occurred with 1 × 106 spores·mL−1, which were added directly to the soil. Spectrophotometry was used for… More >

  • Open Access

    ARTICLE

    Assessment of Phytochemical Analysis, Nutritional Composition and Antimicrobial Activity of Moringa oleifera

    Naima Asghar1, Abida Aziz1, Muhammad Farooq Azhar2 , Mohamed El-Sharnouby3, Uzma Irfan1, Iqra Rafiq1, Husnain Farooq4,5,*, Muhammad Ishaq Asif Rehmani6, Mohamed I. Sakran7, A. Altalhi8, Fahad M. Alzuaibr9, Ayman El Sabagh10,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1817-1829, 2022, DOI:10.32604/phyton.2022.020790

    Abstract Moringa oleifera is a miracle plant rich in nutrients, antioxidants, and antibiotic properties. Present study was designed to evaluate various biochemical attributes of leaves and flowers of M. oleifera. Plant parts (leaves, flowers) of M. oleifera, collected from different roadsides of Multan district, Punjab, Pakistan, were used as experimental material. Result indicates that alkaloids, saponin, carbohydrates, fats, and protein had a high value in the aqueous extract of both leaves and flowers of M. oleifera. Whereas phenol content was high in methanolic leaves extract and the phenol contents were high in aqueous extract of flowers. The extract yield of M.… More >

  • Open Access

    ARTICLE

    Antifungal Activity of Crude Extracts of Tectona grandis L.f. against Wood Decay Fungi

    Enrique Cárcamo-Ibarra1, Mauro Manuel Martínez-Pacheco2, Abril Munro-Rojas1, Jorge Enrique Ambriz-Parra1, Crisanto Velázquez-Becerra1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1795-1808, 2022, DOI:10.32604/phyton.2022.020578

    Abstract Wood is mainly made up of cellulose, hemicelluloses, lignin polymers and other organic and inorganic substances, making it susceptible to deteriorate by various biological agents. Tectona grandis L.f. (Teak) is a timber species with high resistance to biological deterioration, valued for its durability, beauty, and mechanical resistance. The purpose of this work was to evaluate the antifungal activity of crude extracts from teak on various fungi that cause wood deterioration. For this, Teak heartwood was obtained, then fragmented and pulverized until obtaining a flour which was used for compounds extraction using the Soxhlet technique coupled to a rotary evaporator through… More >

  • Open Access

    ARTICLE

    One Step Regioselective Acylation of Polyphenolic Wood Extractive and Its Application for Wood Treatment

    Wissem Sahmim, Georges Eid, Febrina Dellarose Boer, Hubert Chapuis, Philippe Gérardin, Christine Gérardin-Charbonnier*

    Journal of Renewable Materials, Vol.10, No.6, pp. 1491-1503, 2022, DOI:10.32604/jrm.2022.016364

    Abstract This study evaluated the methods of grafting commercial catechin with fatty acids, namely capric acid (C10), lauric acid (C12), and myristic acid (C14) through esterification. Specimens of beech wood (Fagus sylvatica L.) were impregnated with catechin and modified catechin-fatty acids, separately, at a 5% concentration diluted in ethanol using vacuum pressure treatment and subjected to leaching. The weight percentage gain before leaching (WPG), after leaching (WPGAL), and weight loss due to leaching (PL) were investigated. Both leached and unleached samples were tested against white-rot fungi (Trametes versicolor) in Petri-dishes for twelve weeks. Results show that samples treated with modified catechin-fatty… More > Graphic Abstract

    One Step Regioselective Acylation of Polyphenolic Wood Extractive and Its Application for Wood Treatment

  • Open Access

    ARTICLE

    Characterization of Extracts from the Bark of the Gabon Hazel Tree (Coula edulis baill) for Antioxidant, Antifungal and Anti-termite Products

    Christ Stone Arnaud Bopenga Bopenga1, Henri Meyo Degboevi1, Kevin Candelier2, Prosper Edou Engonga3, Stéphane Dumarçay1, Marie France Thévenon2, Christine Gérardin Charbonnier1, Philippe Gérardin1,*

    Journal of Renewable Materials, Vol.9, No.1, pp. 17-33, 2021, DOI:10.32604/jrm.2021.013366

    Abstract

    Chemical composition of the bark extracts of Coula edulis was investigated to find potential antioxidant, anti-termite and antifungal compounds which can find useful applications in the fields of food, nutraceuticals, cosmetics or agrochemical. Phytochemical screening revealed the presence of several groups of active molecules such as alkaloids, polyphenols, flavonoids, saponins and sterols and/or terpenes in the different extracts. Total phenols, condensed tannins and flavonoids contents corroborated phytochemical screening. Gas chromatography-mass spectrometry (GC-MS) analysis revealed compounds in dichloromethane extract different from those obtained with all the other solvents. Hexadecanoic and trans-9-octadecenoic acids, as well as stigmasterol and β-sitosterol have been identified… More >

  • Open Access

    ARTICLE

    Green Synthesis of Silver Nanoparticles from Abronia villosa as an Alternative to Control of Pathogenic Microorganisms

    Ali Abdelmoteleb1, Benjamin Valdez-Salas2, Ernesto Beltran-Partida2, Daniel Gonzalez-Mendoza3,*

    Journal of Renewable Materials, Vol.8, No.1, pp. 69-78, 2020, DOI:10.32604/jrm.2020.08334

    Abstract The aim of this study was to evaluate the antibacterial and antifungal activities of eco-friendly synthesized silver nanoparticles. The silver nanoparticles were synthesized by biological method using aqueous extract of Abronia villosa. Synthesis of silver nanoparticles was confirmed by color change and characterized using UV-visible spectroscopy, scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), and zeta potential analysis. The SEM analysis revealed the presence of spherical silver nanoparticles of the size range 21 to 33 nm. Synthesized silver nanoparticles were used to evaluate their antibacterial effects at different concentrations (25, 50, 75 and 100 µg/ml)… More >

  • Open Access

    ARTICLE

    In vitro evaluation of antifungal activity of Agave (Agave scabra, Salm Dyck) extracts against post-harvest mushrooms

    González-Álvarez M, S Moreno-Limón*, SM Salcedo-Martínez, EC Pérez-Rodríguez

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 427-434, 2015, DOI:10.32604/phyton.2015.84.427

    Abstract The agricultural sector, and particularly the horticultural production, has a singular importance in agriculture, considering that it ranks second on agricultural products, nationally and worldwide. Fungal diseases are one of the major causes of vegetable loss during storage, reducing their nutritional value, quality and sale price. Vegetables are usually exposed to diverse treatments with chemical products before storage; as a result, fungal populations develop an increased resistance over time becoming more difficult to control. Because of this, research efforts toward finding more suitable chemicals to control fungal diseases are needed. Natural extracts may be an alternative solve this problem. In… More >

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