SOHEILA HASANIAN1, NOUR AMIRMOZAFARI2, AHMAD FARAJZADEH SHEIKH3,*, IRAJ MEHREGAN1
BIOCELL, Vol.44, No.3, pp. 411-419, 2020, DOI:10.32604/biocell.2020.08976
- 22 September 2020
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) strains are the essential cause of infections in communities and
hospitals. The present study was conducted to determine the molecular typing of MRSA, isolated from hospitalized patients,
using the double-locus sequence typing (DLST). In total, 280 S. aureus isolated from clinical specimens by phenotypic
(catalase, coagulase, DNase, oxacillin, vancomycin screening agar and antibiotic disk diffusion), and molecular methods
(PCR for determining the mecA, vanA and nuc genes). The DLST and sequencing was performed for MRSA containing
mecA. Out of 280 specimens, confirmed as Staphylococcus aureus (S. aureus), 123 (43.9%) strains were MRSA. The
highest resistance… More >
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