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    ARTICLE

    Identification of differential mRNA and lncRNA expression in AcMNPV-infected Sf9 cells

    TIEJUN ZHAO, RIQIANG DENG*, MENGQIU CHEN, XUNZHANG WANG

    BIOCELL, Vol.46, No.7, pp. 1675-1686, 2022, DOI:10.32604/biocell.2022.018166

    Abstract Sf9Sf9 are the ovarian cells of Spodoptera frugiperda that is the host of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and hence can serve as an effective test vehicle to understand the AcMNPV infection mechanism. In this study, through high-throughput sequencing technology using samples collected from Sf9 cells at different time points after AcMNPV infection, 3463 pieces of time-series differentially expressed RNA (1,200 mRNA and 2,263 lncRNA) are identified and justified by experimental verification of randomly selected samples from them, proving the validity of the bioinformatical analysis on this topic. Functional enrichment analysis and target prediction are performed on those differentially expressed… More >

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