Xianwen Ji^#, Ziying Jiang^#, Jichao Wang, Lili Dong, Xinyi Deng^*

Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 1839-1850, 2024, DOI:10.32604/phyton.2024.052809 - 30 August 2024

Abstract Genes in the glycogen synthase kinase 3 (GSK3) family are essential in regulating plant response to stressful conditions. This study employed bioinformatics to uncover the GSK3 gene family from the sunflower genome database. The expressions of GSK3 genes in different tissues and stress treatments, such as salt, drought, and cold, were assessed using transcriptome sequencing and quantitative real-time PCR (qRT-PCR). The study results revealed that the 12 GSK3 genes of sunflower, belonging to four classes (Classes I–IV), contained the GSK3 kinase domain and 11–13 exons. The majority of GSK3 genes were highly expressed in the leaf axil and… More >

Tongman Zhao^1,2,#, Yujie Fan^1,2,#, Yabing Cao^1,2, Xiaogai Zhao^1,2, Guoqiang Fan^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2473-2488, 2023, DOI:10.32604/phyton.2023.029469 - 28 July 2023

Abstract Somatic embryogenesis receptor-like kinases (SERKs) are receptor-like proteins that contain leucine-rich repeats and are involved in various signaling pathways. This study identified SERK family members in the Paulownia fortunei genome and analyzed their characteristics and expression profiles using bioinformatics methods. We identified 12 SERK genes with relatively conserved gene structures and motifs that were distributed unevenly on eight Paulownia chromosomes. The gene promoters contained various cis-acting elements that regulated the expression of the PfSERK genes in response to hormones and abiotic stresses. Synteny analysis indicated that 10 segmental duplication events had occurred during evolution of the PfSERK family. The More >

Xiaojie Jing^1,2,3,#, Chunyan Li^1,2,3,#, Chengjuan Luo^1,2,3, Chaonan Yao^1,2,3, Jiahao Zhang^1,2,3, Tingting Zhu^1,2,3, Jiuguang Wang^1,2,3, Chaoxian Liu^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 707-723, 2023, DOI:10.32604/phyton.2023.024338 - 29 November 2022

Abstract The zinc finger homeodomain (ZF-HD) genes belong to the homeobox gene family, playing critical roles in flower development and stress response. Despite their importance, however, to date there has been no genome-wide identification and characterization of the ZF-HD genes that are probably involved in stress responses in maize. In this study, 24 ZF-HD genes were identified, and their chromosomal locations, protein properties, duplication patterns, structures, conserved motifs and expression patterns were investigated. The results revealed that the ZF-HD genes are unevenly distributed on nine chromosomes and that most of these genes lack introns. Six and… More >

XIN LI¹, JIANMIN PAN¹, FAISAL ISLAM², JUANJUAN LI¹, ZHUONI HOU¹, ZONGQI YANG¹, LING XU^1,*

BIOCELL, Vol.46, No.8, pp. 1947-1958, 2022, DOI:10.32604/biocell.2022.019806 - 22 April 2022

Abstract NAC (NAM, ATAF, CUC) is a class of transcription factors involved in plant growth regulation, abiotic stress responses, morphogenesis and metabolism. Salvia miltiorrhiza is an important Chinese medicinal herb, but the characterization of NAC genes in this species is limited. In this study, based on the Salvia miltiorrhiza genomic databases, 82 NAC transcription factors were identified, which were divided into 14 groups. Meanwhile, phylogenetic analysis, gene structure, chromosomal localization and potential role of SmNACs in abiotic stress conditions were also studied. The results revealed that some SmNACs had different structures than others, which advised that these genes may have multiple/distinct More >

JIANMIN PAN¹, XIN LI¹, JUANJUAN LI¹, HUI LIU², ZHUONI HOU¹, ZONGQI YANG¹, LING XU^1,*

BIOCELL, Vol.46, No.7, pp. 1751-1762, 2022, DOI:10.32604/biocell.2022.019455 - 17 March 2022

Abstract Plant U-box (PUB) E3 ubiquitin ligases play important roles in hormone signaling pathways and in response to different abiotic stresses, but little is known about U-box genes in Danshen (root of Salvia miltiorrhiza Bunge). Here, we identified and characterized 70 SmPUB genes based on its genome sequence. Phylogenetic analysis of U-box genes from S. miltiorrhiza and Arabidopsis suggested that they can be clustered into seven subgroups (I–VII). Typical U-box domains were found in all identified SmPUB genes through the analysis of conserved motifs. Moreover, qRT-PCR was applied to analyze the relative expression levels of U-box genes in S. miltiorrhiza roots and More >

Misbah Naz¹, Rubab Shabbir^2,17, Krishan K. Verma³, Anshu Rastogi⁴, Vishnu D. Rajput⁵, Talha Javed^2,6, Muhammad Ammar Raza⁷, Kainat Asif⁸, Muhammad Aamir Iqbal⁹, Muhammad Imran¹⁰, Mohammad Sohidul Islam¹¹, Khalid Rehman Hakeem^12,13,*, Mehmet Firat Baran¹⁴, Ayman EL Sabagh^15,16,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 915-927, 2022, DOI:10.32604/phyton.2022.018688 - 24 January 2022

Abstract

Under changing climate, trace elements like selenium (Se) have emerged as vital constituent of agro-ecosystems enabling crop plants to off-set the adverse effects of suboptimal growth conditions. The available form of selenium is important for boosting its bioavailability to crop plants having varied agro-botanical traits and root architectural systems. As compared to selenite, the selenate has a weaker soil bonding, higher absorption in the soil solution which results in a comparatively absorption by plant roots. Various factors including dry climate, high pH, optimal ambient air temperature, less accumulation of water, and low concentration of organic

… More >

Seerat Saleem¹, Naveed Ul Mushtaq¹, Wasifa Hafiz Shah¹, Aadil Rasool¹, Khalid Rehman Hakeem^2,*, Reiaz Ul Rehman^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1363-1385, 2021, DOI:10.32604/phyton.2021.014826 - 27 May 2021

Abstract Abiotic stresses such as drought, heat, cold, nutrient deficiency, excess salt and hazardous metals can hamper plantgrowth and development. Intensive agriculture of only a few major staple food crops that are sensitive and intolerant to environmental stresses has led to an agrarian crisis. On the other hand, nutritionally rich, gluten free and stress tolerant plants like millets are neglected and underutilized. Millets sustain about one-third of the world’s population and show exceptional tolerance to various abiotic and biotic stresses. Millets are C4 plants that are adapted to marginal and dry lands of arid and semi-arid… More >

QICHAO WANG¹, WUJING ZENG¹, BASHARAT ALI², XUEMIN ZHANG³, LING XU^1,*, ZONGSUO LIANG^1,*

BIOCELL, Vol.45, No.4, pp. 1107-1119, 2021, DOI:10.32604/biocell.2021.015282 - 22 April 2021

Abstract Andrographis paniculata (A. paniculata) is a Chinese herbal medicine that clears away heat, reduces inflammation, protects the liver, and promotes choleretics. The WRKYs of A. paniculata are still not well characterized, although many WRKYs have been identified in various plant species. In the present study, 59 A. paniculata WRKY (ApWRKY) genes were identified and renamed on the basis of their respective chromosome distribution. These ApWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and combined with WRKY of Arabidopsis. The 59 identified ApWRKY transcription factors were nonuniformity distributed on 23 chromosomes of A. paniculata. From the structural… More >

MST FARHANA NAZNEEN CHOWDHURY¹, MOHD RAFII YUSOP^1,2,*, SITI IZERA ISMAIL³, SHAIRUL IZAN RAMLEE¹, YUSUFF OLADOSU², MONIR HOSEN², GOUS MIAH⁴

BIOCELL, Vol.44, No.3, pp. 269-278, 2020, DOI:10.32604/biocell.2020.09627 - 22 September 2020

Abstract Chili (Capsicum annuum L.) is the popular spicy vegetable crops belonging to family Solanaceae. Chili peppers are known for their pungency characteristic due to the presence of capsaicinoids that classifies them into hot or sweet pepper. Chili is used as spices, folk remedies for diseases, vegetables, and coloring agent showing a diverse role in human’s life. However, its production is hampered by different biotic stress and abiotic factors. Similarly, the unavailability of high yielding varieties, high temperature, and disease incidence, particularly, anthracnose disease, are the major constraints responsible for the low production of chili pepper. The… More >

Lu Zhu, Qiuyue Ma, Shushun Li, Jing Wen, Kunyuan Yan, Qianzhong Li^*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 385-403, 2020, DOI:10.32604/phyton.2020.09259 - 22 April 2020

Abstract Quantitative real-time reverse transcriptase PCR (qRT-PCR) technology has been extensively used to estimate gene expression levels, and the selection of appropriate reference genes for qRT-PCR analysis is critically important for obtaining authentic normalized data. Acer palmatum is an important colorful leaf ornamental tree species, and reference genes suitable for normalization of the qRT-PCR data obtained from this species have not been investigated. In this study, the expression stability of ten candidate reference genes, namely, Actin3, Actin6, Actin9, EF1α, PP2A, SAMDC, TIP41, TUBα, TUBβ and UBQ10, in two distinct tissues (leaves and roots) of A. palmatum under four different abiotic stress conditions (cold,… More >