JINJIN FU1,#, XIAOYUE FENG2,#, JUAN WEI2, XIANG GENG1, YU GONG1, FENGDONG LI1, SHAOHUA ZHUANG1, JIN HUANG1, FANGYU WANG2,*
BIOCELL, Vol.47, No.12, pp. 2627-2639, 2023, DOI:10.32604/biocell.2023.042586
- 27 December 2023
Abstract Background: This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells (BM-MSCs) may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid (TNBS), specifically through autophagy modulation. Methods: BM-MSCs were collected from BALB/c mice for subsequent experiments. The study employed cell counting kits (CCK-8) to investigate the impact of the MSC-conditioned medium (M medium) on the proliferation of RAW264.7 macrophages. The GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic flux. The gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction (qRT-PCR). Western blot… More >
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