Tengyu Li, Jing Zhu, Shuai Zuo, Shanwen Chen, Ju Ma, Yongchen Ma, Shihao Guo,
Pengyuan Wang, Yucun Liu
Oncology Research, Vol.27, No.7, pp. 739-750, 2019, DOI:10.3727/096504018X15360541345000
Abstract The activated form of vitamin D3, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], regulates numerous cellular
processes, including inhibition of cancer progression. IL-1 has been reported to facilitate cancer development, especially by inducing an epithelial-to-mesenchymal transition (EMT) in several malignant tumors.
However, the underlying mechanism of 1,25(OH)2D3 and IL-1 in colorectal cancer (CRC) still remains
largely unknown. To fill in this knowledge gap, we measured cell proliferation and invasion by CCK-8 and
Transwell assays after stimulation with 1,25(OH)2D3 and IL-1 . E-cadherin and vimentin were chosen as
markers of EMT measured by immunofluorescence, quantitative real-time PCR (qRT-PCR), and Western
blot. The expression and function… More >
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