Paul Chege^1,2, Erzsébet Kiss², Csaba Lantos¹, Andrea Palágyi¹, János Pauk^1,2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 475-487, 2021, DOI:10.32604/phyton.2021.013557 - 07 February 2021

Abstract Sorghum [Sorghum bicolor (L.) Moench] can benefit from accelerated breeding and release of improved varieties through doubled haploid technology. The technology has been used in speeding up the breeding of other major cereals such as wheat, maize and rice, for which generally widely applied optimised protocols exist. A reproducible protocol for the crop, that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however lacking. This study aimed at sorghum doubled haploids production thereby contributing to the development of an improved protocol. From the 28 hybrid genotypes, both F₁ registered- and experimental hybrids… More >

Paul Chege^1,2, Andrea Palágyi², Csaba Lantos², Erzsébet Kiss¹, János Pauk^2,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 111-119, 2020, DOI:10.32604/phyton.2020.07554 - 01 March 2020

Abstract Many attempts on optimization of sorghum [Sorghum bicolor (L.) Moench] tissue culture induction media have been made, but the culture system remains with some bottlenecks compared to that of other crops. This study aimed at assessing the suitability of various induction media to produce embryogenic callus (yellow and friable) with high induction rates and reduced phenolic exudation. The six culture medium modifications: 3 based on Murashige and Skoog (MS) medium and one each based on Chu N6, Gamborg B5 and 190-2 media respectively were applied in the culture of mature embryos from 10 sorghum genotypes. Although… More >

Espinoza-Sánchez EA¹, YA Sánchez-Peña², JA Torres-Castillo³, EA García-Zambrano², JE Treviño Ramírez², F Zavala-García², SR Sinagawa-García²

Phyton-International Journal of Experimental Botany, Vol.87, pp. 105-112, 2018, DOI:10.32604/phyton.2018.87.105

Abstract In this study, we established an in vitro regeneration system from immature embryos of four varieties of Sweet Sorghum with biotechnological potential. A total of 17 treatments were assesses in the induction of somatic embryogenic calli. We analyzed the effect of Murashige y Skoog media supplemented with different concentrations of plant growth regulators such as auxins and cytokinins. The callus induction response was evaluated using three immature embryos development stages and based on the number of embryos with callus induction. Different responses were observed in the embryos depending of the embryo age, culture condition, media… More >

Castro-Nava S¹, J Ortiz-Cereceres^2,†, M del C Mendoza-Castillo², AJ Huerta³

Phyton-International Journal of Experimental Botany, Vol.81, pp. 149-156, 2012, DOI:10.32604/phyton.2012.81.149

Abstract The aim of this study was to determine the efficiency of drought classification criteria that we previously reported for our grain sorghum genotypes. Two tolerant genotypes of grain sorghum [Sorghum bicolor (L.) Moench] (UAT-124 and UAT-152) and one susceptible (UAT-30) were subjected to drought under greenhouse conditions at either panicle initiation, flag leaf, or flowering. Results showed that the effects of drought depended on when drought stress occurred during development. Biomass of the three sorghum genotypes was significantly reduced at the flag leaf stage (48%) by drought stress, but the greatest reduction occurred in the susceptible… More >

Sadia^1,3 B, PC Josekutty², SD Potlakayala², P Patel², S Goldman³, SV Rudrabhatla²

Phyton-International Journal of Experimental Botany, Vol.79, pp. 177-181, 2010, DOI:10.32604/phyton.2010.79.177

Abstract A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded More >