Yuting Luan1, Cong Xu1, Xiaoxiao Wang1, Daqiu Zhao1, Jun Tao1,2,*
Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 1045-1059, 2022, DOI:10.32604/phyton.2022.018767
- 24 January 2022
Abstract The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy. Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors. In this study, the expression profiles of ten candidate genes, ACT1, ACT2, GAPDH, eIF1, eIF2, α-TUB, β-TUB, TBP, RNA Pol II and RP II, were calculated for a suitable reference gene selection in Paeonia ostii T. Hong et J. X. Zhang leaves under various drought stress conditions. Data were processed by the four regularly used evaluation software. A comprehensive analysis revealed More >
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