MINGHONG WEI1,#, CHENG XIAO1,2,#, JIAN WU1,3, LIHONG QIN1,3, HONGLIANG LIU1,3, YANG CAO1,*, YUMIN ZHAO1,3,*
BIOCELL, Vol.47, No.9, pp. 2115-2123, 2023, DOI:10.32604/biocell.2023.030364
- 28 September 2023
Abstract Background: The regulatory genes related to lipid metabolism affect the intramuscular fat (IMF) content and improve meat quality traits. Mediator Complex Subunit 4 (MED4), a vitamin D receptor protein, affects vitamin D, livestock growth, carcass traits, and triglyceride deposition. However, the physiological function of the MED4 gene on bovine adipocyte differentiation remains unknown. Methods: This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe cattle. The overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using More >
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