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  • Open Access

    ARTICLE

    Rapid Identification of a Candidate Gene Related to Fiber Strength Using a Superior Chromosome Segment Substitution Line from Gossypium hirsutum × Gossypium barbadense via Bulked Segregant RNA-Sequencing

    Qi Zhang1,#, Pengtao Li2,#, Aiying Liu1, Shaoqi Li1, Quanwei Lu2, Qun Ge1, Junwen Li1, Wankui Gong1, Xiaoying Deng1, Haihong Shang1,3, Yuzhen Shi1,*, Youlu Yuan1,3,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 837-858, 2021, DOI:10.32604/phyton.2021.014437 - 30 March 2021

    Abstract Cotton is the most widely cultivated commercial crop producing natural fiber around the world. As a critical trait for fiber quality, fiber strength principally determined during the secondary wall thickening period. Based on the developed BC5F3:5 CSSLs (chromosome segment substitution lines) from Gossypium hirsutum CCRI36 × G. barbadense Hai 1, the superior MBI9915 was chosen to construct the secondary segregated population BC7F2 with its recurrent parent CCRI36, which was subsequently subjected to Bulk segregant RNA-sequencing (BSR-seq) for rapid identification of candidate genes related to fiber strength. A total of 4 fiber-transcriptome libraries were separately constructed and sequenced, including… More >

  • Open Access

    ARTICLE

    Dynamic Expression Analysis and Introgressive Gene Identification of Fiber Length Using Chromosome Segment Substitution Lines from G. hirsutum × G. barbadense

    Pengtao Li1,2,#,*, Quanwei Lu1,#, Xianghui Xiao3, Rui Yang3, Xixi Duan1

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 129-144, 2021, DOI:10.32604/phyton.2021.012928 - 20 November 2020

    Abstract Fiber length is a critical trait that principally determines cotton spinning quality, while Upland cotton as the most widely cultivated Gossypium species around the world subjects to the relatively ordinary fiber performance. Chromosome segment substitution lines (CSSLs) have been introduced in cotton breeding to take full advantages of superior fiber quality and high yield from Sea Island and Upland cotton, respectively, which serve as ideal materials for elucidating the genetic mechanism of complex quantitative traits. Here, three CSSLs derived from CCRI45 (G. hirsutum) × Hai1 (G. barbadense), two superior (MBI7561 and MBI7747) and one (MBI7285) with ordinary… More >

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