Liying Gong^1,2,3, Hongkun Jiang⁴, Guangrong Qiu¹, Kailai Sun^1,*

Congenital Heart Disease, Vol.16, No.5, pp. 499-512, 2021, DOI:10.32604/CHD.2021.015831 - 03 June 2021

Abstract Background: microRNAs are crucial for cardiovascular development and are associated with congenital heart disease (CHD). Recent studies have shown that microRNAs play a role in heart development and is closely related to CHD. The present study investigated the underlying mechanism of microRNA-208a (miR-208a) in “simple” CHD. Material and Methods: Reverse transcription-quantitative PCR (RT-qPCR) demonstrated miR-208a expression levels in children with CHD (n = 27) compared with normal controls (n = 29), in cardiomyocytes from embryo 10 (E10) to post-birth (P7) and organs in adult rats in healthy rats. Apoptosis of H9c2 cells after transfection with miR-208a detected… More >

Fariborz Soheili^1,2, Zahra Jalili³, Mahtab Rahbar⁴, Zahed Khatooni¹, Amir Mashayekhi⁵, Hossein Jafari⁶

Congenital Heart Disease, Vol.13, No.2, pp. 295-304, 2018, DOI:10.1111/chd.12571

Abstract Background: The mutations in GATA4 gene induce inherited atrial and ventricular septation defects, which is the most frequent forms of congenital heart defects (CHDs) constituting about half of all cases.
Method: We have performed High resolution melting (HRM) mutation scanning of GATA4 coding exons of nonsyndrome 100 patients as a case group including 39 atrial septal defects (ASD), 57 ventricular septal defects (VSD) and four patients with both above defects and 50 healthy individuals as a control group. Our samples are categorized according to their HRM graph. The genome sequencing has been done for 15 control samples and… More >