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    ARTICLE

    In Vitro Propagation and Artificial Seed Production of Fritillaria cirrhosa D. Don, an Endangered Medicinal Plant

    Qian Tao, Guiqi Han, Bujin Ma, Hongmei Jia, Can Zhao, Wenshang Li, Zhuyun Yan*

    Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1297-1310, 2024, DOI:10.32604/phyton.2024.051923 - 27 June 2024

    Abstract Fritillaria cirrhosa D. Don (Liliaceae) is an endangered perennial bulbous plant and its dry bulb is a valuable medicinal material with antitussive and expectorant effects. Nevertheless, lack of resources and expensive prices make it difficult to meet clinical needs. This study presents a regeneration system aimed at overcoming the challenge of inadequate supply in F. cirrhosa, focusing on: (1) callus induction, (2) bulblets and adventitious bud induction, and (3) artificial seed production. Callus development was achieved in 84.93% on Murashige and Skoog (MS) medium fortified with 1.0 mg·L picloram. The optimal medium for callus differentiation into regenerated… More >

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