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  • Open Access

    ARTICLE

    Computational Microfluidic Channel for Separation of Escherichia coli from Blood-Cells

    Chinnapalli Likith Kumar1,*, A. Vimala Juliet1, Bandaru Ramakrishna2, Shubhangi Chakraborty1, Mazin Abed Mohammed3, Kalakanda Alfred Sunny4

    CMC-Computers, Materials & Continua, Vol.67, No.2, pp. 1369-1384, 2021, DOI:10.32604/cmc.2021.015116 - 05 February 2021

    Abstract Microfluidic channels play a vital role in separation of analytes of interest such as bacteria and platelet cells, etc., in various biochemical diagnosis procedures including urinary tract infections (UTI) and bloodstream infections. This paper presents the multi physics computational model specifically designed to study the effects of design parameters of a microfluidics channel for the separation of Escherichia coli (E. coli) from various blood constituents including red blood cells (RBC) and platelets. A standard two inlet and a two outlet microchannel of length 805 m with a channel width of 40 m is simulated. The effect of… More >

  • Open Access

    ARTICLE

    Detection of new antibiotic resistance gene profile in Escherichia coli associated with avian leukosis virus infection from broiler chickens

    HAIFENG WANG1,2,*, JUAN GUO1, LIJING ZHENG1, SHUYING LIU1, ZHERONG WANG1, HONGXUAN HE2

    BIOCELL, Vol.44, No.2, pp. 217-224, 2020, DOI:10.32604/biocell.2020.09051 - 27 May 2020

    Abstract The Escherichia coli (E. coli) is prevailing worldwide, but the epidemiology of E. coli infections feature regional distribution characteristics to some extent. E. coli, as a zoonotic pathogen, can be transferred from animals to humans through food chain or via contact with wounds, causing a public health risk. We reported the swelling of proventriculus and tracheal bleeding following the death in two broiler chickens (Gallus gallus domesticus) from Beijing, China. To investigate whether a virus was involved in the infection, Madin Darby Bovine Kidney (MDCK) cells were co-cultured with supernatants of proventriculus, trachea and spleen homogenates. The avian leucosis virus… More >

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