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    ARTICLE

    Effect of Putrescine on Low-Temperature Acclimation in Chlamydomonas reinhardtii

    Muslum S. Inal1,2, Dilek Unal1,*, Bengu Turkyilmaz Unal3,*, Munir Ozturk4

    Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 583-598, 2022, DOI:10.32604/phyton.2022.018223 - 26 October 2021

    Abstract Putrescine is reported to be necessary for cold acclimation under low-temperature stress. In this study, the effect of low-temperature on some physiological and biochemical parameters has been investigated using the green algae Chlamydomonas reinhardtii. The lipid peroxidation rate, amount of Rubisco protein, activities of antioxidant enzymes and gene expression of polyamine biosynthesis (odc2, and spd1), heat shock proteins (hsp70c, hsp90a, and hsp90c), and PSII repair mechanisms (psba, rep27, and tba1) were determined to understand the low-temperature response. Exogenous putrescine application significantly increased Rubisco protein concentration and catalase enzyme activities under low-temperature stress. Moreover, real-time RT-PCR results and gene expression analysis… More >

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