Zongmin Mou1,2,3, Fei Ye4, Fangchen Shen1, Dake Zhao1,2,3,*
Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2331-2348, 2022, DOI:10.32604/phyton.2022.021273
- 30 May 2022
Abstract Aconitum vilmorinianum, a well-known traditional Chinese herb, is recently being threatened by overexploitation
and environment disturbance. This study was conducted to provide propagation methods through in vitro germination and explant cultivation. Germination was stimulated up to 66.00% on Murashige and Skoog (MS) medium containing 2.0 mg L−1 6-benzylaminopurine (BAP), 0.1 mg L−1 1-napthaleneacetic acid (NAA), and 30 g L−1
sucrose. Three bacteria (Pantoea agglomerans, Erwinia persicina, and Pseudomonas tolaasii) would be responsible
for consistent contamination during germination. The latter two were effectively eradicated after disinfected. The
influence of explant types and hormone combinations on direct and indirect organogenesis was evaluated in the… More >
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