Longgang Wang^2,3,#, Xiangxun Zhuansun^2,3,#, Yao Li^2,3, Qili Yao^2,3, Qi Liu^2,3,*, Huijing Lin^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 441-454, 2025, DOI:10.32604/phyton.2025.061720 - 06 March 2025

Abstract Fructus Ligustri Lucidi (FLL) refers to the dried mature fruit of Ligustrum lucidum Ait., a species from the Oleaceae family, widely distributed across East Asia and India. This study aimed to optimize the extraction process for Fructus Ligustri Lucidi essential oil (FLLO) to develop an efficient and practical extraction method. Additionally, the chemical composition of FLLO was analyzed, and its antioxidant, antimicrobial, and cytotoxic activities were evaluated. FLLO was extracted using supercritical CO₂ extraction, and response surface methodology was applied to optimize the extraction parameters: pressure of 16 MPa, temperature of 40°C, and extraction time of 40… More > Graphic Abstract

Jalil Ahmad^1,*, Muhammad Munir^2,*, Nashi Alqahtani^2,3, Tahira Alyas⁴, Muhammad Ahmad⁵, Sadia Bashir⁶, Fasiha Qurashi⁷, Abdul Ghafoor⁸, Hassan Ali–Dinar²

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 281-302, 2025, DOI:10.32604/phyton.2025.061534 - 06 March 2025

Abstract A steady rise in the overall population is creating an overburden on crops due to their global demand. On the other hand, given the current climate change and population growth, agricultural practices established during the Green Revolution are no longer viable. Consequently, innovative practices are the prerequisite of the time struggle with the rising global food demand. The potential of nanotechnology to reduce the phytotoxic effects of these ecological restrictions has shown significant promise. Nanoparticles (NPs) typically enhance plant resilience to stressors by fortifying the physical barrier, optimizing photosynthesis, stimulating enzymatic activity for defense, elevating More >

Cheng Sun^1,3, Chen Zhao^2,3, Guohua Wang^2,3, Qianwen Mao^2,3, Rong Han^2,3,*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 455-479, 2025, DOI:10.32604/phyton.2025.061462 - 06 March 2025

Abstract Enhanced UV-B radiation represents a major environmental factor impacting global cereal production. Researchers have explored various approaches to reduce the detrimental impact of UV-B radiation on crops. Recently, engineered nanoparticles, particularly cerium oxide nanoparticles (CeO₂-NPs), have attracted widespread interest for their ability to boost plant tolerance to a range of abiotic stresses. This study investigates how CeO₂-NPs application affects the morphology, physiology, biochemistry, and transcriptomics profiles of wheat seedling roots subjected to enhanced UV-B stress. The findings demonstrate that CeO₂-NPs notably promoted root length, fresh and dry weights, and root activity (p < 0.05) under enhanced UV-B… More >

Wentao Sheng^*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 331-345, 2025, DOI:10.32604/phyton.2025.061424 - 06 March 2025

Abstract Pyrola atropurpurea Franch is an important annual herbaceous plant. Few genomic analyses have been conducted on this plant, and chloroplast genome research will enrich its genomics basis. This study is based on high-throughput sequencing technology and Bioinformatics methods to obtain the sequence, structure, and other characteristics of the P. atropurpurea chloroplast genome. The result showed that the chloroplast genome of P. atropurpurea has a double-stranded circular structure with a total length of 172,535 bp and a typical four-segment structure. The genome has annotated a total of 132 functional genes, including 43 tRNAs, 8 rRNAs, 76 protein-coding genes, and… More >

Sha Luo¹, Shuang Wu¹, Junyan Song¹, Xuena Yu¹, Kehao Liang¹, Zhi Huang¹, Yi Tang¹, Huanxiu Li¹, Victor Hugo Escalona², Zhifeng Chen³, Fen Zhang^1,*, Bo Sun^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 521-538, 2025, DOI:10.32604/phyton.2025.061099 - 06 March 2025

Abstract This study conducted karyotype analysis on 56 Chinese kale varieties from Guangdong and Fujian provinces using conventional chromosome analysis methods. The varieties were categorized into four groups based on their origin and flower color: white-flower Chinese kale originating from Guangdong (GW), yellow-flower Chinese kale originating from Guangdong (GY), white-flower Chinese kale originating from Fujian (FW), and yellow-flower Chinese kale originating from Fujian (FY). Karyotype differences among the four groups of Chinese kale were analyzed, and the evolutionary relationship between yellow-flower and white-flower Chinese kale from the two regions was inferred based on karyotype parameters. The… More >

Xianfeng Zhou^1,2,*, Ruiju Sun¹, Zhaojie Zhang¹, Yuanyuan Song¹, Lijiao Jin¹, Lin Yuan³

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 503-519, 2025, DOI:10.32604/phyton.2025.060827 - 06 March 2025

Abstract An accurate and robust estimation of leaf chlorophyll content (LCC) is very important to better know the process of material and energy exchange between plants and the environment. Compared with traditional remote sensing methods, abundant research has made progress in agronomic parameter retrieval using different CNN frameworks. Nevertheless, limited reports have paid attention to the problems, i.e., limited measured data, hyperspectral redundancy, and model convergence issues, when concerning CNN models for parameter estimation. Therefore, the present study tried to analyze the effects of synthetic data size expansion employing a Gaussian process regression (GPR) model for… More >

Xiuyun Liu¹, Jixia Sun², Feng Ming³, Minxiao Liu^2,*, Xinyu Wang², Yingjie Zhang^2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 379-391, 2025, DOI:10.32604/phyton.2025.060726 - 06 March 2025

Abstract To study the main aroma components of Phalaenopsis orchid and their relationship with colors, 10 fragrant cultivars with different colors, like pink, rose, yellow, and purple, were used as samples in this experiment. Headspace-gas chromatography-mass spectrometry was used to determine the main components of floral fragrance and analyze the correlation between floral color and fragrance. The results showed that the main aroma components of the 10 fragrant cultivars of Phalaenopsis were alcohols, alkenes, esters, and benzene ring compounds, and the main aroma components of different cultivars were diverse. The main aroma components of yellow fragrant flowers… More >

Qi Sheng¹, Huiqin Ma^1,*, Jingcheng Zhang¹, Zhiqin Gui¹, Wenjiang Huang^2,3, Dongmei Chen¹, Bo Wang¹

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 421-440, 2025, DOI:10.32604/phyton.2025.060152 - 06 March 2025

Abstract Yellow rust (Puccinia striiformis f. sp. Tritici, YR) and fusarium head blight (Fusarium graminearum, FHB) are the two main diseases affecting wheat in the main grain-producing areas of East China, which is common for the two diseases to appear simultaneously in some main production areas. It is necessary to discriminate wheat YR and FHB at the regional scale to accurately locate the disease in space, conduct detailed disease severity monitoring, and scientific control. Four images on different dates were acquired from Sentinel-2, Landsat-8, and Gaofen-1 during the critical period of winter wheat, and 22 remote sensing features that… More >

Ibrahim Soe^1,2, Emmanuel Odama³, Alex Tamu^1,2, Aquilino Lado Legge Wani^1,4, Taiichiro Ookawa⁵, Abdelbagi M. Ismail⁶, Jun-Ichi Sakagami^1,7,*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 303-316, 2025, DOI:10.32604/phyton.2025.060016 - 06 March 2025

Abstract Rice varieties tolerant to submergence regulate shoot elongation during short-term submergence by expressing the SUB1A gene. In contrast, the deep-rooted DRO1 is effectively expressed under drought conditions to enhance water and nutrient uptake. This study investigates the growth and yield of rice with both SUB1A and DRO1 in the background of IR64, under early-season flooding, and mid-season drought. The study used a randomized complete design with two factors: soil moisture treatments (submergence, drought, and their combination) and genotypes. The genotypes included IR64, and three near-isogenic lines (NILs): NIL-SUB1DRO1, NIL-SUB1, and NIL-DRO1. Complete submergence was imposed for 7… More >

Amine Batbat^1,2, Mohamed El Fadili³, Naoufal El Hachlafi^4,*, Amine Elbouzidi^5,*, Chaymae Hmimen⁶, Hiba Yahyaoui^2,7, Samir Jeddi⁶, Abdellatif Benbouazza², Kawtar Fikri-Benbrahim⁶, Mohamed Addi⁵, Samiah Hamad Al-Mijalli⁸, Khaoula Habbadi², Hassane Greche¹

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 481-501, 2025, DOI:10.32604/phyton.2025.059841 - 06 March 2025

Abstract This study highlights the regulatory potential antibacterial and antiradical of Origanum elongatum essential oil (EO), an endemic medicinal plant of Morocco used for its various properties. The chemical composition of the EO was characterized using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity against different agricultural phytopathogens was determined by disc diffusion and microatmosphere methods, as well as by the determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC), while the antioxidant activity was evaluated by DPPH and FRAP assays. To complement the experimental analyses, a molecular docking approach was used to predict and elucidate… More >