Open Access

ARTICLE

Research on the Cymbidium tortisepalum var. longibracteatum Growth and Non-Tube Rapid Propagation Based on Response Surface Methodology

Guolan Wang, Ting Xie, Lijun Fu, Siying Qu, Jie Li^*

College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621000, China

* Corresponding Author: Jie Li. Email:

(This article belongs to the Special Issue: Optimization of Propagation Techniques and Improvement of the Production and Quality of Red Fruit)

Phyton-International Journal of Experimental Botany 2025, 94(3), 953-971. https://doi.org/10.32604/phyton.2025.060634

Received 06 November 2024; Accepted 08 February 2025; Issue published 31 March 2025

Abstract

The objective of this study was to determine the optimal proportions of plant growth regulators for growth and non-tube rapid propagation of Cymbidium tortisepalum var. longibracteatum; seedlings were utilized as the material. The effects of various combinations and concentrations of 6-benzylaminopurine (6-BA), gibberellic acid (GA₃), and naphthaleneacetic acid (NAA) on growth and non-tube rapid propagation were assessed through a single-factor testing and response surface methodology. The results indicated that 6-BA at 60 mg/L, GA₃ at 150 mg/L, and NAA at 30 mg/L were the most effective concentrations for promoting leaf buds formation in the single-factor analysis. Response surface methodology clarified the sensitivity of the proliferation rate of lateral buds to the three factors, with 6-BA being the most influential, followed by GA₃ and NAA. The increase in leaf area was most significantly influenced by NAA, then GA₃, and least by 6-BA, while the increase in plant height was most responsive to GA₃, followed by 6-BA, and then NAA. The ideal concentrations of plant growth regulators were established as 6-BA at 43 mg/L, GA₃ at 169 mg/L, and NAA at 36 mg/L. Under these conditions, the lateral bud number per plant was 2.78, with a leaf area increment of 2.87 cm² and a plant height increment of 2.67 cm.

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Keywords

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