Open Access

ARTICLE

Genome-Wide Identification of ABCC Gene Subfamily Members and Functional Analysis of CsABCC11 in Camellia sinensis

by Mingyuan Luo¹, Shiyu Tian¹, Xinzhuan Yao², Yue Wan⁴, Zhouzhuoer Chen¹, Zifan Yang⁴, Huagen Hao⁴, Fei Liu³, Hu Tang^1,2,*, Litang Lu^1,2,*

1 The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Science, Guizhou University, Guiyang, 550025, China
2 College of Tea Science, Institute of Plant Health & Medicine, Guizhou University, Guiyang, 550025, China
3 Wengfu Group, Guiyang, 550025, China
4 Huaneng Clean Energy Research Institute, Guiyang, 550000, China

* Corresponding Authors: Hu Tang. Email: ; Litang Lu. Email:

(This article belongs to the Special Issue: Plant Secondary Metabolism and Functional Biology)

Phyton-International Journal of Experimental Botany 2024, 93(8), 2019-2036. https://doi.org/10.32604/phyton.2024.052938

Received 19 April 2024; Accepted 15 July 2024; Issue published 30 August 2024

Abstract

The ATP-binding cassette (ABC) transporter is a gene superfamily in plants. ATP-binding cassette subfamily C (ABCC) protein is a multidrug resistance-associated (MRP) transporter. They play various roles in plant growth, development, and secondary metabolite transport. However, there are few studies on ABCC transporters in tea plants. In this study, genome-wide association study (GWAS) analysis of epigallocatechin gallate (EGCG) content in 108 strains of Kingbird revealed that CsABCCs may be involved in EGCG transport. We identified 25 CsABCC genes at the genomic level of the tea plant, their phylogenetic tree, gene structure, targeted miRNA and other bioinformatics were analyzed. The expression patterns of CsABCCs in eight different tissues and abiotic stress indicate that they have potential roles in regulating the growth, development, and defense of tea plants. The correlation analysis revealed that the expression of the CsABCC11 gene was closely related to the EGCG content in tea buds of 108 strains of the Kingbird, and the subcellular localization experiments in tobacco showed that CsABCC11 protein was localized on the plasma membrane. The virus-induced gene silencing (VIGS) strategy in tea plants further verified that CsABCC11 was involved in EGCG accumulation. Our study laid a foundation for studying the biological function of CsABCC and provided a new candidate molecular marker gene for further EGCG-related variety breeding, which will be of great interest to breeders.

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Supplementary Material

Supplementary Material File

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