Open Access

ARTICLE

Monitoring Xylem Transport in the Stem of Lilium lancifolium Using Fluorescent Dye 5(6)-Carboxyfluorescein Diacetate

by Yulin Luo^1,2,#, Panpan Yang^2,#, Mengmeng Bi², Leifeng Xu², Fang Du^3,*, Jun Ming^2,*

1 College of Horticulture, Shanxi Agricultural University, Jinzhong, 030801, China
2 State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
3 College of Urban and Rural Construction, Shanxi Agricultural University, Jinzhong, 030801, China

* Corresponding Authors: Fang Du. Email: ; Jun Ming. Email:

(This article belongs to the Special Issue: Botany, Phytochemistry and Biological Properties of Medicinal and Aromatic Plants )

Phyton-International Journal of Experimental Botany 2024, 93(5), 1057-1066. https://doi.org/10.32604/phyton.2024.051197

Received 29 February 2024; Accepted 12 April 2024; Issue published 28 May 2024

Abstract

The xylem undergoes physiological changes in response to various environmental conditions during the process of plant growth. To understand these physiological changes, it is extremely important to observe the transport of xylem. In this study, the distribution and structure of vascular bundle in Lilium lancifolium were observed using the method of semithin section. Methods for introducing a fluorescent tracer into the xylem of the stems were evaluated. Then, the transport rule of 5(6)-Carboxyfluorescein diacetate (CFDA) in the xylem of the stem of L. lancifolium was studied by fluorescence dye in live cells tracer technology. The results showed that the vascular bundles of L. lancifolium were scattered in the basic tissue, the peripheral vascular bundles were smaller and densely distributed, and the closer to the center, the larger the volume of vascular bundles and the more sparsely distributed. The vascular bundles of L. lancifolium are limited external tenacity vascular bundles, which are composed of phloem and xylem. The most suitable method for CFDA labeling the xylem of isolated stem segments of L. lancifolium was solution soaking for 24 h. The running speed of CF in the isolated stem was 0.3 cm/h, which was consistent with the running speed of the material in the field. CF could be transported between the xylem and parenchyma cells, indicating that the material transport in the xylem could be through the symplastic pathway. The above results laid a foundation for the study of the xylem transport mechanism and the xylem pathogen disease of lily.

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Keywords

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