Open Access

ARTICLE

Effects of Potassium-Solubilizing Bacteria on Growth, Antioxidant Activity and Expression of Related Genes in Fritillaria taipaiensis P. Y. Li

Jiaqi Lang¹, Mingyan Ye¹, Ya Luo¹, Yueheng Wang¹, Zhifen Shi^1,2, Xiaotian Kong^1,3, Xuan Li¹, Nong Zhou^1,*

1 Chongqing Engineering Laboratory of Green Planting and Deep Processing of Famous-Region Drug in the Three Gorges Reservoir Region, College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing, 404120, China
2 College of Modern Chinese Medicine Industry, Chengdu University of Traditional Chinese Medicine, Chengdu, 611130, China
3 College of Pharmacy, Dali University, Dali, 671000, China

* Corresponding Author: Nong Zhou. Email:

(This article belongs to the Special Issue: Microbial Biotechnology Applications on Plant Performance and Physiology for Sustainable Production )

Phyton-International Journal of Experimental Botany 2024, 93(4), 789-806. https://doi.org/10.32604/phyton.2024.049088

Received 27 December 2023; Accepted 19 March 2024; Issue published 29 April 2024

Abstract

This study aimed to examine the effects of inoculating Fritillaria taipaiensis P.Y.Li leaves with different strains of potassium-solubilizing bacteria (KSB), or combinations thereof, focusing on aspects of photosynthesis and physiological and biochemical characteristics. At present, some studies have only studied the rhizosphere microbial community characteristics of F. taipaiensis and have not discussed the effects of different microbial species on the growth promotion of F. taipaiensis. This paper will start from the perspective of potassium-solubilizing bacteria to conduct an in-depth study. Seed cultivation commenced at the base with three different KSBs in early October 2022. The growth of F. taipaiensis leaves was observed after different treatments. Both single-plant and compound inoculations were executed. A total of eight treatment groups were established, with aseptic fertilizer and sterilized soil functioning as the control group. The results reveal that intercellular CO concentration (Ci), stomatal conductance (Gs), and transpiration rate (Tr) were at their apex in the S7 group. Most treatment groups exhibited an increase in leaf area, photosynthetic pigment content, soluble sugar, soluble protein, Superoxide Dismutase (SOD), Peroxidase (POD), Catalase (CAT) activities, and proline content. The expression levels of POD, SOD, and CAT genes were evaluated, following inoculation with different KSB. The highest was the S7 group. The inoculation with various KSB, or combinations thereof, appears to bolster the growth and development of F. taipaiensis. The composite inoculation group S7, comprising Bacillus cereus, Burkholderia cepacia, and Bacillus subtilis, manifested the most favorable impact on the diverse indices of F. taipaiensis, thereby furnishing valuable insights for the selection of bacterial fertilizer in the artificial cultivation of F. taipaiensis.

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Keywords

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