Open Access

ARTICLE

Analysis and Verification of the Conserved MYB Binding Element in the DFR Promoter in Compositae

Jialei Guo^1,2, Fengzhen Li², Guomin Shi³, Weimin Zhao², Tao He^1,2,*

1 State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, China
2 School of Ecol-Environmental Engineering, Qinghai University, Xining, 810016, China
3 Key Laboratory of Landscape Plants of Qinghai Province, Qinghai University, Xining, 810016, China

* Corresponding Author: Tao He. Email:

(This article belongs to the Special Issue: Plant Secondary Metabolism and Functional Biology)

Phyton-International Journal of Experimental Botany 2024, 93(2), 343-353. https://doi.org/10.32604/phyton.2024.047429

Received 05 November 2023; Accepted 10 January 2024; Issue published 27 February 2024

Abstract

Anthocyanins, ubiquitous in the Compositae family, are regulated by MYB (v-myb avian myeloblastosis viral oncogene homolog), playing an important role in anthocyanin synthesis. In this study, we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase (DFR) expression in Compositae, and validated this law in Saussurea medusa through yeast one-hybrid experiments. Our results showed that MYB and DFR underwent purification selection, DFR promoter analysis revealed the presence of MYB binding site (GAGTTGAATGG) and bHLH binding site (CANNTG) at the sense strand of 84–116 nucleotide residues from the start codon. These two motifs were separated by 9–10 nucleotide residues, as existed in the DFR promoters of many Compositae plants. Furthermore, the yeast one-hybrid experiment demonstrated that SmMYB1 can activate the promoter of SmDFR. Our results provide a reference for further functional study of DFR in Compositae.

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Keywords

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Supplementary Material

Supplementary Material File

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