Open Access
ARTICLE
Analysis and Verification of the Conserved MYB Binding Element in the DFR Promoter in Compositae
Jialei Guo1,2, Fengzhen Li2, Guomin Shi3, Weimin Zhao2, Tao He1,2,*
1 State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, China
2 School of Ecol-Environmental Engineering, Qinghai University, Xining, 810016, China
3 Key Laboratory of Landscape Plants of Qinghai Province, Qinghai University, Xining, 810016, China
* Corresponding Author: Tao He. Email:
(This article belongs to the Special Issue: Plant Secondary Metabolism and Functional Biology)
Phyton-International Journal of Experimental Botany 2024, 93(2), 343-353. https://doi.org/10.32604/phyton.2024.047429
Received 05 November 2023; Accepted 10 January 2024; Issue published 27 February 2024
Abstract
Anthocyanins, ubiquitous in the Compositae family, are regulated by MYB (v-myb avian myeloblastosis viral oncogene homolog), playing an important role in anthocyanin synthesis. In this study, we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase (DFR) expression in Compositae, and validated this law in
Saussurea medusa through yeast one-hybrid experiments. Our results showed that MYB and DFR underwent purification selection,
DFR promoter analysis revealed the presence of MYB binding site (GAGTTGAATGG) and bHLH binding site (CANNTG) at the sense strand of 84–116 nucleotide residues from the start codon. These two motifs were separated by 9–10 nucleotide residues, as existed in the
DFR promoters of many Compositae plants. Furthermore, the yeast one-hybrid experiment demonstrated that SmMYB1 can activate the promoter of
SmDFR. Our results provide a reference for further functional study of
DFR in Compositae.
Keywords
Supplementary Material
Supplementary Material File
Cite This Article
APA Style
Guo, J., Li, F., Shi, G., Zhao, W., He, T. (2024). Analysis and verification of the conserved MYB binding element in the dfr promoter in compositae. Phyton-International Journal of Experimental Botany, 93(2), 343-353. https://doi.org/10.32604/phyton.2024.047429
Vancouver Style
Guo J, Li F, Shi G, Zhao W, He T. Analysis and verification of the conserved MYB binding element in the dfr promoter in compositae. Phyton-Int J Exp Bot. 2024;93(2):343-353 https://doi.org/10.32604/phyton.2024.047429
IEEE Style
J. Guo, F. Li, G. Shi, W. Zhao, and T. He "Analysis and Verification of the Conserved MYB Binding Element in the DFR Promoter in Compositae," Phyton-Int. J. Exp. Bot., vol. 93, no. 2, pp. 343-353. 2024. https://doi.org/10.32604/phyton.2024.047429