Open Access

ARTICLE

A New Micropropagation Technology of Tilia amurensis: In Vitro Micropropagation of Mature Zygotic Embryos and the Establishment of a Plant Regeneration System

by Shijie Lin¹, Zimo Wang¹, Hongbo Zhu², Conghui Wang³, Hongfeng Wang², Dawei Zhang¹, Tianbing Gou¹, Guangdao Bao¹, Ye Luo¹, Huaijiang He¹, Zhonghui Zhang^1,*

1 Jilin Provincial Academy of Forestry Sciences (Jilin Forestry Biological Control Central Station), Changchun, 130033, China
2 Institute of Forestry Inventory and Planning of Jilin Province, Changchun, 130022, China
3 Jilin Forest Tree and Seeding Management Station, Changchun, 130022, China

* Corresponding Author: Zhonghui Zhang. Email:

Phyton-International Journal of Experimental Botany 2024, 93(2), 277-289. https://doi.org/10.32604/phyton.2024.046989

Received 21 October 2023; Accepted 29 December 2023; Issue published 27 February 2024

Abstract

Tilia amurensis is an economically valuable broadleaf tree species in Northeast China. The production of high-quality T. amurensis varieties at commercial scales has been greatly limited by the low germination rates. There is thus a pressing need to develop an organogenesis protocol for in vitro propagation of T. amurensis to alleviate a shortage of high-quality T. amurensis seedlings. Here, we established a rapid in vitro propagation system for T. amurensis from mature zygotic embryos and analyzed the effects of plant growth regulators and culture media in different stages. We found that Woody plant medium (WPM) was the optimal primary culture medium for mature zygotic embryos. The highest callus induction percentage (68.76%) and number of axillary buds induced (3.2) were obtained in WPM + 0.89 µmol/L 6-benzyladenine (6-BA) + 0.46 µmol/L kinetin (KT) + 0.25 µmol/L indole-3-butryic acid (IBA) + 1.44 µmol/L gibberellin A3 (GA₃). The multiple shoot bud development achieved the highest percentage (83.32%) in the Murashige and Skoog (MS) + 2.22 µmol/L 6-BA + 0.25 µmol/L IBA + 1.44 µmol/L GA₃. The rooting percentage (96.70%) was highest in 1/2 MS medium + 1.48 µmol/L IBA. The survival percentage of transplanting plantlets was 82.22% in soil:vermiculite:perlite (5:3:1). Our study is the first to establish an effective organogenesis protocol for T. amurensis using mature zygotic embryos.

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