Open Access

ARTICLE

Genome-Wide Identification and Expression Pattern Analysis of LONELY GUY Gene Family in Walnut (Juglans regia)

by Yuan Wang¹, Tianle Zhang¹, Xinfeng Zeng¹, Jiale Liu¹, Siyu Li¹, Siyu Yang¹, Shengnan Zhao¹, Abdullah Shah², Muhammad Saif Ullah², Guohui Qi^1,*, Peng Jia^1,*

1 State Key Laboratory of North China Crop Improvement and Regulation/College of Forestry, Hebei Agricultural University, Baoding, 071000, China
2 Department of Agriculture, Abdul Wali Khan University, Mardan, 23200, Pakistan

* Corresponding Authors: Guohui Qi. Email: ; Peng Jia. Email:

(This article belongs to the Special Issue: Integrative Approaches to Plant Stress Responses under Changing Climate Conditions)

Phyton-International Journal of Experimental Botany 2024, 93(12), 3331-3346. https://doi.org/10.32604/phyton.2024.059402

Received 07 October 2024; Accepted 21 November 2024; Issue published 31 December 2024

Abstract

LONELY GUY (LOG) is a pivotal cytokinin-activating enzyme that plays an important role in plant growth, development, and stress responses. Walnut (Juglans regia), an important woody oilseed species, has not yet undergone systematic identification of its LOG gene family. In this study, we identified 17 JrLOG genes in the walnut genome, which are unevenly distributed across 11 chromosomes. JrLOG gene expansion was primarily driven by gene duplication, along with purifying selection. Members of the JrLOG family were categorized into five groups, each exhibiting analogous gene structures, featured motifs, and conserved domains. Transcriptome and quantitative real-time PCR analyses revealed that JrLOG gene expression was tissue-specific, developmentally regulated, and responsive to stress situations. Notably, JrLOG3, which localizes to the cell membrane, exhibited high expression levels in leaves and responded to both cold and pathogen infection treatments. This study represents the first comprehensive identification of the LOG gene family in walnut, offering essential data for further functional studies of this gene family.

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Keywords

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Supplementary Material

Supplementary Material File

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