Open Access

ARTICLE

Identification and Molecular Characterization of the Alkaloid Biosynthesis Gene Family in Dendrobium catenatum

Liping Yang^1,#, Xin Wan^2,3,#, Runyang Zhou¹, Yingdan Yuan^1,*

1 College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China
2 Jiangsu Academy of Forestry, Nanjing, China
3 Jiangsu Yangzhou Urban Forest Ecosystem National Observation and Research Station, Yangzhou, China

* Corresponding Author: Yingdan Yuan. Email:
# These authors contributed equally

Phyton-International Journal of Experimental Botany 2024, 93(1), 81-96. https://doi.org/10.32604/phyton.2023.045389

Received 25 August 2023; Accepted 22 November 2023; Issue published 26 January 2024

Abstract

As one of the main active components of Dendrobium catenatum, alkaloids have high medicinal value. The physicochemical properties, conserved domains and motifs, phylogenetic analysis, and cis-acting elements of the gene family members in the alkaloid biosynthesis pathway of D. catenatum were analyzed by bioinformatics, and the expression of the genes in different years and tissues was analyzed by qRT-PCR. There are 16 gene families, including 25 genes, in the D. catenatum alkaloid biosynthesis pathway. The analysis of conserved domains and motifs showed that the types, quantities, and orders of domains and motifs were similar among members of the same family, but there were significant differences among families. Phylogenetic analysis indicated that the gene family members showed some evolutionary conservation. Cis-acting element analysis revealed that there were a large number of light-responsive elements and MYB (v-myb avian myeloblastosis viral oncogene homolog)-related elements in these genes. qRT-PCR showed that expressions of gene family members involved in alkaloid synthesis were different in different years and tissues of D. catenatum. This study provides a theoretical basis for further exploration of the regulatory mechanisms of these genes in the alkaloid biosynthesis of D. catenatum.

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Keywords

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Supplementary Material

Supplementary Material File

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