Open Access

ARTICLE

Genome-Wide Analysis of the F3′5′H Gene Family in Blueberry (Vaccinium corymbosum L.) Provides Insights into the Regulation of Anthocyanin Biosynthesis

by Xiaolan Guo^1,2, Jinbin Hu³, Shimei Yang⁴, Delu Wang^2,*, Jianbing Wang¹

1 College of Life Sciences, Huizhou University, Huizhou, 516007, China
2 College of Forestry, Guizhou University, Guiyang, 550025, China
3 School of Architecture and Civil Engineering, Huizhou University, Huizhou, 516007, China
4 Guizhou Institute of Subtropical Crop., Guizhou Academy of Agricultural Sciences, Xingyi, 562400, China

* Corresponding Author: Delu Wang. Email:

Phyton-International Journal of Experimental Botany 2023, 92(9), 2683-2697. https://doi.org/10.32604/phyton.2023.029820

Received 10 March 2023; Accepted 08 June 2023; Issue published 28 July 2023

Abstract

The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis, abiotic stress, and hormone signaling. In this study, 14 F3′5′H genes were identified from the blueberry genome. The chromosomal distribution, physicochemical properties, F3′5′H domain, conserved motifs, cis-acting elements, and intron/exon compositions were analyzed. The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites. The results of qRT-PCR showed that VcF3′5′Hs (especially VcF3′5′H4) were highly expressed at the ripening stage. Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum. Co-expression analysis showed that the VcF3′5′H gene family was related to anthocyanin. This research provides an overview of the blueberry F3′5′H family and helps verify the role of these genes in regulating anthocyanin biosynthesis.

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Keywords

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Supplementary Material

Supplementary Material File

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