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Somatic embryogenesis receptor-like kinases (SERKs) are receptor-like proteins that contain leucine-rich repeats and are involved in various signaling pathways. This study identified SERK family members in the Paulownia fortunei genome and analyzed their characteristics and expression profiles using bioinformatics methods. Many PfSERK genes can respond to drought and salt stress. Combined with RNA-seq and protein interaction network, it is speculated that PfSERK3/11 may participate in the occurrence of Paulownia witches' broom (PaWB) by regulating the plant height of Paulownia.

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  • Open AccessOpen Access

    ARTICLE

    Identification of the SERK Gene Family in Paulownia fortunei and Its Involvement in the Response to Biotic and Abiotic Stresses

    Tongman Zhao1,2,#, Yujie Fan1,2,#, Yabing Cao1,2, Xiaogai Zhao1,2, Guoqiang Fan1,2,*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2473-2488, 2023, DOI:10.32604/phyton.2023.029469 - 28 July 2023
    (This article belongs to the Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)
    Abstract Somatic embryogenesis receptor-like kinases (SERKs) are receptor-like proteins that contain leucine-rich repeats and are involved in various signaling pathways. This study identified SERK family members in the Paulownia fortunei genome and analyzed their characteristics and expression profiles using bioinformatics methods. We identified 12 SERK genes with relatively conserved gene structures and motifs that were distributed unevenly on eight Paulownia chromosomes. The gene promoters contained various cis-acting elements that regulated the expression of the PfSERK genes in response to hormones and abiotic stresses. Synteny analysis indicated that 10 segmental duplication events had occurred during evolution of the PfSERK family. The More >

  • Open AccessOpen Access

    ARTICLE

    Evaluation of Beta-Lactam Antibiotics on the Regeneration of Peanut Plants and Their Inhibitory Effect on Agrobacterium Growth

    Abraham Lamboro1,3,*, Songnan Yang1, Xueying Li1, Dan Yao2, Jun Zhang1,*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2489-2501, 2023, DOI:10.32604/phyton.2023.029492 - 28 July 2023
    Abstract The effect of beta-lactam antibiotics on shoot induction and plantlet regeneration from cotyledonary nodes was tested using two peanut cultivars. The culture media contained 4 mg/L 6-benzylaminopurine (BAP) as the main growth regulator. Various concentrations (100–600 mg/L) of cefotaxime, carbenicillin, and timentin were applied in the culture media. In all the tested media, there were no significant differences in the shoot induction as compared to the control. However, little phytotoxic effect was observed at higher concentrations of these antibiotics in the shoot elongation media. Under shoot elongation medium, shoots turned brownish and partly died at… More >

  • Open AccessOpen Access

    ARTICLE

    Effects of Plant Extracts and Beauveria bassiana on the Activity of Defense-Related Enzymes in Solanum lycopersicum L. during Interaction with Fusarium oxysporum f. sp. lycopersici

    José Adrian Perez-Robles, Carlos Alberto Lecona-Guzmán*, Víctor Manuel Ruíz-Valdiviezo, Joaquín Adolfo Montes-Molina*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2503-2518, 2023, DOI:10.32604/phyton.2023.029784 - 28 July 2023
    Abstract The objective to this work was to evaluate the enzymatic activity in the culture of Solanum lycopersicum L. infected with Fusarium oxysporum after the combined application of Beauveria bassiana and plant extracts. Solanum lycopersicum plantlets were transplanted 15 days after the emergency. Five days after transplanting, Beauveria bassiana spores were applied at a concentration of 1 × 107 spores mL−1 onto soil (along with A. indica (N) and P. auritum (H) leaf extracts) where S. lycopersicum plants were planted. Eight days after transplanting, spores of F. oxysporum strain were applied at a concentration of 1 × 106 spores mL−1 to soil where S. lycopersicum plants were growing.… More >

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    ARTICLE

    A Novel Hybrid Model Based on Machine and Deep Learning Techniques for the Classification of Microalgae

    Volkan Kaya1, İsmail Akgül1, Özge Zencir Tanır2,*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2519-2534, 2023, DOI:10.32604/phyton.2023.029811 - 28 July 2023
    Abstract Classification and monitoring of microalgae species in aquatic ecosystems are important for understanding population dynamics. However, manual classification of algae is a time-consuming method and requires a lot of effort with expertise due to the large number of families and genera in its classification. The recognition of microalgae species has become an increasingly important research area in image recognition in recent years. In this study, machine learning and deep learning methods were proposed to classify images of 12 different microalgae species in order to successfully classify algae cells. 8 Different novel models (MobileNetV3Small-Lr, MobileNetV3SmallRf, MobileNetV3Small-Xg,… More >

  • Open AccessOpen Access

    ARTICLE

    Comparative Transcriptome Analysis of Seed Germination of a Cotton Variety with High Tolerance to Low Temperature

    Genhai Hu1,*, Maoni Chao1, Xiuren Zhou2, Yuanzhi Fu2
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2535-2554, 2023, DOI:10.32604/phyton.2023.030163 - 28 July 2023
    Abstract Gossypium hirsutum L. is an important cash crop native to the subtropics and is widely cultivated around the world. Low temperature is an important stress that seriously affects seed germination and emergence during planting. In this study, transcriptomic profiles of low-temperature- and normal-temperature-germinated seeds of Xinluzao 25, a variety with low-temperature tolerance and high germination rates, were analyzed and compared. The following results were obtained. (1) A total of 81.06 Gb of clean data were obtained after transcriptome sequencing and assembly, and 76,931 non-redundant Unigene sequences were obtained after data consolidation and concatenation; of these, 69,883… More >

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    ARTICLE

    Transcriptome and Metabolome Revealed the Mechanism of NtBRL3 Overexpression Tobacco (Nicotiana tabacum L. K326) in Response to Drought Stress

    Jing Yang, Tianxiunan Pu, Ke Wan, Linqi Wang, Yuanshuai Shi, Xu Luo, Jie Tan, Dongmei Wang, Yang Liu*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2555-2576, 2023, DOI:10.32604/phyton.2023.030301 - 28 July 2023
    (This article belongs to the Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)
    Abstract Drought has severely affected the yield and quality of commercial crops. The BRI1 family plays an important role in plant response to drought stress, and BRL3 gene plays an important role in the study of drought in Arabidopsis thaliana. In this study, NtBRL3 was constructed as a vector and genetically transformed to obtain ‘N. Tobacco K326’ overexpression of NtBRL3. The enzyme activities of transgenic tobacco and wild-type tobacco were measured and transcriptome and metabolome analyses were performed. The results showed that the antioxidant enzymes of transgenic tobacco were more active under drought conditions, and 85 significantly differentially metabolites and More >

  • Open AccessOpen Access

    ARTICLE

    Quantitative Trait Loci (QTL) Mapping and Marker Analysis of Fatty Acids in Peanut

    Xiao Han, Songnan Yang, Xueying Li, Qiulin Wu, Yongyi Xing, Jun Zhang*, Fenglou Ling*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2577-2589, 2023, DOI:10.32604/phyton.2023.029440 - 28 July 2023
    (This article belongs to the Special Issue: Integrating Agronomy and Plant Physiology for Improving Crop Production)
    Abstract Peanut, with high oil content, has been a major oil and food crop globally. The compositions of the fatty acids are the common factors in determining the oil quality. In the present study, an F2 segregated population with 140 individuals derived from the cross of Weihua8 (a cultivar) and 12L49 (a line with high oleic acid concentration) was used to construct a genetic map and conduct QTL mapping analysis. A total of 103 polymorphic SSR primers were utilized for genotyping the RILs and finally generating the SSR loci. Within the 103 SSR loci, a genetic… More >

  • Open AccessOpen Access

    ARTICLE

    Transcriptome Profiling of the Salt-Stress Response in Paper Mulberry

    Jie Zhang1, Yingwei Zhao2, Hongying Li3, Jianwei Ni4,*, Dongmei Wang1,*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2591-2610, 2023, DOI:10.32604/phyton.2023.028338 - 28 July 2023
    (This article belongs to the Special Issue: Development of New Sensing Technology in Sustainable Farming and Smart Environmental Monitoring)
    Abstract Paper mulberry is a high-quality woody feed resource plant with high crude protein content. It is widely distributed in China and has excellent characteristics of salt and alkali tolerance. Paper mulberry has ecological and economic importance. Salt stress has become a critical factor with the increasing degree of soil salinity that restricts plant growth. In the saline-alkali environments, transcriptome expression is altered leading to phenotypic defects in most plants. However, the regulatory mechanism related to paper mulberry’s salt-stress (SS) response is not clearly understood. In the present study de novo transcriptomic assembly was performed, and gene… More >

  • Open AccessOpen Access

    ARTICLE

    Quick and Accurate Counting of Rapeseed Seedling with Improved YOLOv5s and Deep-Sort Method

    Chen Su, Jie Hong, Jiang Wang, Yang Yang*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2611-2632, 2023, DOI:10.32604/phyton.2023.029457 - 28 July 2023
    (This article belongs to the Special Issue: Development of New Sensing Technology in Sustainable Farming and Smart Environmental Monitoring)
    Abstract The statistics of the number of rapeseed seedlings are very important for breeders and planters to conduct seed quality testing, field crop management and yield estimation. Calculating the number of seedlings is inefficient and cumbersome in the traditional method. In this study, a method was proposed for efficient detection and calculation of rapeseed seedling number based on improved you only look once version 5 (YOLOv5) to identify objects and deep-sort to perform object tracking for rapeseed seedling video. Coordinated attention (CA) mechanism was added to the trunk of the improved YOLOv5s, which made the model… More >

  • Open AccessOpen Access

    ARTICLE

    Retrieval of Winter Wheat Canopy Carotenoid Content with Ground- and Airborne-Based Hyperspectral Data

    Ting Cui, Xianfeng Zhou*, Yufeng Huang, Yanting Guo, Yunrui Lin, Leyi Song, Jingcheng Zhang
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2633-2648, 2023, DOI:10.32604/phyton.2023.029259 - 28 July 2023
    Abstract Accurate assessment of canopy carotenoid content (CCx+cC) in crops is central to monitor physiological conditions in plants and vegetation stress, and consequently supporting agronomic decisions. However, due to the overlap of absorption peaks of carotenoid (Cx+c) and chlorophyll (Ca), accurate estimation of carotenoid using reflectance where carotenoid absorb is challenging. The objective of present study was to assess CCx+cC in winter wheat (Triticum aestivum L.) with ground- and aircraft-based hyperspectral measurements in the visible and near-infrared spectrum. In-situ hyperspectral reflectance were measured and airborne hyperspectral data were acquired during major growth stages of winter wheat in five consecutive… More >

  • Open AccessOpen Access

    ARTICLE

    TCD5 Enhances the Photosynthesis Capacity, Increases the Panicle Number and the Yield in Rice

    Jing Yang1,2, Yufeng Wang2, Zhanghua Hu3, Xiaoping Chen4, Yanjun Dong5,*, Sheng Teng1,*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2649-2663, 2023, DOI:10.32604/phyton.2023.030710 - 28 July 2023
    (This article belongs to the Special Issue: High-Yield Rice Physiology & Genetics)
    Abstract Improvement of photosynthetic efficiency is a major approach to increase crop yield potential. Previously, we cloned a gene encoding the chloroplast-located putative monooxygenase TCD5, which is essential in plastid development under low temperature in rice (Oryza sativa L.). In this study, the effects of TCD5 on the photosynthesis and the yields were investigated in rice. Two sets of genetic materials with three levels of TCD5 expression, including tcd5 mutant or TCD5 RNAi transgenic lines and TCD5 over-expression transgenic lines in Jiahua1 and Nipponbare backgrounds, were used in the field trails of multi-locations and multi-years. TCD5 positively affected the panicle… More >

  • Open AccessOpen Access

    REVIEW

    Molecular Mechanism Underlying Plant Response to Cold Stress

    Yiwei Cao, Delight Hwarari, Yasmina Radani, Yuanlin Guan, Liming Yang*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2665-2683, 2023, DOI:10.32604/phyton.2023.024929 - 28 July 2023
    Abstract Low temperature stress is one of the most important factors limiting plant growth and geographical distribution. In order to adapt to low temperature, plants have evolved strategies to acquire cold tolerance, known as, cold acclimation. Current molecular and genomic studies have reported that annual herbaceous and perennial woody plants share similar cold acclimation mechanisms. However, woody perennials also require extra resilience to survive cold winters. Thus, trees have acquired complex dynamic processes to control the development of dormancy and cold resistance, ensuring successful tolerance during the coldest winter season. In this review, we systemically described More >

  • Open AccessOpen Access

    ARTICLE

    Genome-Wide Analysis of the F3′5′H Gene Family in Blueberry (Vaccinium corymbosum L.) Provides Insights into the Regulation of Anthocyanin Biosynthesis

    Xiaolan Guo1,2, Jinbin Hu3, Shimei Yang4, Delu Wang2,*, Jianbing Wang1
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2683-2697, 2023, DOI:10.32604/phyton.2023.029820 - 28 July 2023
    Abstract The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis, abiotic stress, and hormone signaling. In this study, 14 F3′5′H genes were identified from the blueberry genome. The chromosomal distribution, physicochemical properties, F3′5′H domain, conserved motifs, cis-acting elements, and intron/exon compositions were analyzed. The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites. The results of qRT-PCR showed that VcF3′5′Hs (especially VcF3′5′H4) were highly expressed at the ripening stage. Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum. Co-expression analysis showed that the VcF3′5′H More >

  • Open AccessOpen Access

    ARTICLE

    Inoculation of Chlorella and Food Waste Improves the Physio-Morphological Features of Red Pepper by Regulating Activating Antioxidant Defense System

    Sang-Mo Kang1,#, Shifa Shaffique1,#, Muhammad Imran2,#, Su-Mi Jeon3, Shabir Hussain Wani5, Muhammad Aaqil Khan4, Peter Odongkara1, Eun-Hae Kwon1, Yosep Kang1, Joon-Ik Son6, Won-Chan Kim1,*, In-Jung Lee1,*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2699-2711, 2023, DOI:10.32604/phyton.2023.028224 - 28 July 2023
    (This article belongs to the Special Issue: Comprehensive Effects of Biochar or other Additives after Applied to Agricultural and Forest Soils)
    Abstract Food waste is recognized as a valuable source for potential agricultural applications to supply organic matter and nutrients to arable soil. However, the information on the combined application of food waste and the plant growth-promoting bacterial strain, Chlorella, related to plant metabolic features and sodium chloride content in arable soil is limited. The present study was conducted to investigate the exogenous application of food waste along with Chlorella, which improved the physio-morphological features of red pepper. Our results revealed that this combination enhanced the organic matter in the soil, ultimately improving the fertility rate of the More >

  • Open AccessOpen Access

    REVIEW

    Breaking Barriers: Selenium and Silicon-Mediated Strategies for Mitigating Abiotic Stress in Plants

    Mojtaba Kordrostami1, Ali Akbar Ghasemi-Soloklui1, Mohammad Anwar Hossain2,*, Mohammad Golam Mostofa3,4,*
    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2713-2736, 2023, DOI:10.32604/phyton.2023.030372 - 28 July 2023
    (This article belongs to the Special Issue: Selenium, Silicon and their Nanoparticles-mediated Environmental Stress Tolerance in Crop Plants)
    Abstract Numerous plant species, particularly those that can accumulate selenium (Se) and silicon (Si), benefit from these essential micronutrients. Se and Si accumulation in plants profoundly affects several biochemical reactions in cells. Understanding how plants react to Se/Si enrichment is crucial for ensuring adequate dietary Se/Si intake for humans and animals and increasing plant tolerance to environmental stressors. Several studies have shown that Se/Si-enriched plants are more resistant to salinity, drought, extreme temperatures, UV radiation, and excess metalloids. The interplay between Se/Si in plants is crucial for maintaining growth and development under normal conditions while providing… More >

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