Open Access

ARTICLE

In Vitro Synthetic Seed Production of Potato under Different Fungicide Levels and Storage Intervals

Tahira Kalsoom¹, Touqeer Ahmed¹, Muhammad Azam Khan¹, Mirza Hasanuzzaman^2,*, Mukhtar Ahmed^3,*, Stefaan P. O. Werbrouck⁴

1 Department of Horticulture, PMAS-Arid Agriculture University, Rawalpindi, 46300, Pakistan
2 Department of Agronomy, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh
3 Department of Agronomy, PMAS-Arid Agriculture University, Rawalpindi, 46300, Pakistan
4 Department of Plants and Crops, Ghent University, Ghent, 9000, Belgium

* Corresponding Authors: Mirza Hasanuzzaman. Email: ; Mukhtar Ahmed. Email:

Phyton-International Journal of Experimental Botany 2023, 92(8), 2429-2450. https://doi.org/10.32604/phyton.2023.030846

Received 09 February 2023; Accepted 04 May 2023; Issue published 25 June 2023

Abstract

Potato propagation through tubers and seed is an inefficient way of propagation thus we propose an alternative method in this study which is in vitro propagation. Plantlets were raised from True Potato Seeds (TPS) in test tubes containing 10 mL of Murashige Skoog (MS) medium. Subculturing was done after every 21 days and after four subsequent subculturing, when there was an adequate quantity of stock material, uninodal cuttings of about 3–4 mm in length were prepared from in vitro proliferated potato plants by excising leaves of 1–2 mm on each side of the node. Prepared uninodal cuttings were cultured on ½ strength MS medium for root primordia development. The selected uninodal cuttings with root primordia were encapsulated with sodium alginate solution (3.5% (w/v), mixed with 1.25% charcoal and different concentration of fungicide (thiophanate methyl, sigma) to enhance the viability percentage of synthetic seed. Furthermore, these encapsulated uninodal cuttings were subjected to different storage intervals to check maximum storage. Results showed that optimum dose for fungicide is 150 mg/L as it resulted in good viability percentage, shoot number, shoot length, rooting percentage, root number, root length and ideal for bead formation with sodium alginate. Similarly, results revealed that encapsulated uninodal cuttings can be stored at a temperature of 4°C up to 45 days with a survival rate of 63.33%. Encapsulated uninodal cutting stored for 60 days showed a poor viability percentage of about 43.03%. It can be concluded that optimum dose of 150 mg/L with storage time of 45 days should be used to get a better outcome for synthetic potato seed production.

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Keywords

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