Open Access

ARTICLE

Effects of Manganese on the Antioxidant System and Related Gene Expression Levels in the “Hong Yang” Kiwifruit Seedlings

Chongpei Zheng¹, Liangliang Li², Zhencheng Han², Weijie Li^2,*, Xiaopeng Wen^1,*

1 Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-Bioengineering, College of Life Sciences, Guizhou University, Guiyang, 550025, China
2 Institute of Mountain Resource, Guizhou Academy of Sciences, Guiyang, 550001, China

* Corresponding Authors: Weijie Li. Email: ; Xiaopeng Wen. Email:

(This article belongs to the Special Issue: New Approaches to Mitigate Abiotic and Biotic Stresses for Improving Crop Productivity and Quality)

Phyton-International Journal of Experimental Botany 2023, 92(8), 2399-2412. https://doi.org/10.32604/phyton.2023.029891

Received 14 March 2023; Accepted 27 April 2023; Issue published 25 June 2023

Abstract

To explore how manganese affects the antioxidant system and the expression levels of related genes of “Hong yang” seedlings, the leaves of its tissue cultured seedlings were taken as test materials, and single factor treatment was performed by changing the manganese chloride (MnCl₂^.4H₂O) solution concentration when spraying the leaves. The expression levels of Mn-SOD, POD64 and POD27 genes in leaves were quantitatively analyzed by real-time quantitative PCR (qRT-PCR) at different determination times. Meanwhile, the contents of malondialdehyde (MDA), hydrogen peroxide (H₂O₂), the activities of antioxidant enzymes, including catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). The results showed that the SOD, CAT, POD, ascorbate peroxidase (APX), and reduced glutathione (GSH) activities in leaves were the highest at 12 h post-treatment with 50 μM MnCl₂·4H₂O. Furthermore, the contents of MDA and H₂O₂ in leaves also peaked when the concentration of H₂O₂ is 50 μM, which is the minimum value. Additionally at 50 μM Mn²⁺, the Mn-SOD and POD27 expression was up-regulated as compared to the control, which promoted the expression of their respective enzyme activities. However, POD64 expression increased with the increasing Mn²⁺ concentration. Therefore, 50 μM is the optimal concentration of Mn when exogenously applied on “Hong yang”, which improve the antioxidant enzyme activity and regulate the plant’s physiological and biochemical functions.

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Keywords

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